1887

Abstract

The effect of various lectins and sugars on adhesion of five strains of to buccal and vaginal epithelial was investigated. Adhesion of GDH 2346 was inhibited primarily by -fucose and winged-pea lectin, whereas adhesion of strain GDH 2023 was inhibited by -acetyl--glucosamine, or -glucosamine, and wheat-germ agglutinin. Three other strains of (MRL 3153, GRI 681 and GRI 682) gave results similar to those obtained with strain GDH 2346. Extracellular polymeric material (EP) isolated from strain GDH 2346 inhibited adhesion of strains MRL 3153, GRI 681 and GRI 682 by more than 50%, but that of strain GDH 2023 by only 30%. EP from strain GDH 2023 had little or no effect on the adhesion of any other yeast strain. Lectin-like proteins with affinities for -fucose, -acetyl--glucosamine and -mannose were detected in EP from all five strains in different amounts. These results indicate that there are at least two types of adhesion mechanism and that glycosides containing -fucose or -acetyl--glucosamine can function as epithelial cell receptors for .

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1987-03-01
2024-04-24
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References

  1. Burnie J.P., Odds F.C., Lee W., Webster C., Williams J.D. 1985; Outbreak of systemic Candida albicans in intensive care unit caused by cross infection. British Medical Journal 290:746–748
    [Google Scholar]
  2. Centeno A., Davis C.P., Cohen M.S., Warren M.M. 1983; Modulation of Candida albicansattachment to human epithelial cells by bacteria and carbohydrates. Infection and Immunity 39:1354–1360
    [Google Scholar]
  3. Cinco M., Banfi E., Ruaro E., Crevatin D., Crotti D. 1984; Evidence for L-fucose (6-deoxy-l-galactopyranose)-mediated adherence of Campylobacter spp. to epithelial cells. FEMS Microbiology Letters 21:347–351
    [Google Scholar]
  4. Collins-Lech C., Kalbfleisch J.H., Franson T.R., Sohnle P.G. 1984; Inhibition by sugars of Candida albicans adherence to human buccal mucosal cells and corneocytes in vitro. Infection and Immunity 46:831–834
    [Google Scholar]
  5. Critchley I.A., Douglas L.J. 1987; Isolation and partial characterization of an adhesin from Candida albicans. . Journal of General Microbiology 133:629–636
    [Google Scholar]
  6. Diamond R.D., Krzesicki R. 1978; Mechanisms of attachment of neutrophils to Candida albicanspseudohyphae in the absence of serum, and of subsequent damage to pseudohyphae by microbicidal processes of neutrophils in vitro. Journal of Clinical Investigation 61:360–369
    [Google Scholar]
  7. Douglas L.J. 1985; Adhesion of pathogenic Candida species to host surfaces. Microbiological Sciences 2:243–247
    [Google Scholar]
  8. Douglas L.J. 1987; Adhesion to surfaces. In The Yeasts, 2nd edn.. 2 pp 239–280 Rose A. H., Harrison J. S. Edited by London: Academic Press;
    [Google Scholar]
  9. Douglas L.J., Houston J.G., Mccourtie J. 1981; Adherence of Candida albicans to human buccal epithelial cells after growth on different carbon sources. FEMS Microbiology Letters 12:241–243
    [Google Scholar]
  10. Jones G.W., Freter R. 1976; Adhesive properties of Vibrio cholerae: nature of the interaction with isolated rabbit brush border membranes and human erythrocytes. Infection and Immunity 14:240–245
    [Google Scholar]
  11. Jones G.W., Isaacson R.E. 1983; Proteinaceous bacterial adhesins and their receptors. CRC Critical Reviews in Microbiology 10:229–260
    [Google Scholar]
  12. Lee J.C., King R.D. 1983; Characterization of Candida albicans adherence to human vaginal epithelial cells in vitro. Infection and Immunity 41:1024–1030
    [Google Scholar]
  13. Makrides H.C., Macfarlane T.W. 1983; An investigation of the factors involved in increased adherence of C. albicans to epithelial cells mediated by E. coli. . Microbios 38:177–185
    [Google Scholar]
  14. Mccourtie J., Douglas L.J. 1981; Relationship between cell surface composition of Candida albicansand adherence to acrylic after growth on different carbon sources. Infection and Immunity 32:1234–1241
    [Google Scholar]
  15. Mccourtie J., Douglas L.J. 1984; Relationship between cell surface composition, adherence and virulence of Candida albicans. . Infection and Immunity 45:6–12
    [Google Scholar]
  16. Reinhart H., Muller G., Sobel J.D. 1985; Specificity and mechanism of in vitro adherence of Candida albicans. . Annals of Clinical and Laboratory Science 15:406–413
    [Google Scholar]
  17. Rotrosen D., Edwards J.E. Jr Gibson T.R., Moore J.C., Cohen A.H., Green I. 1985; Adherence of Candida to cultured vascular endothelial cells: mechanisms of attachment and endothelial cell penetration. Journal of Infectious Diseases 152:1264–1274
    [Google Scholar]
  18. Sandin R.L., Rogers A.L., Patterson R.J., Beneke E.S. 1982; Evidence for mannose-mediated adherence of Candida albicans to human buccal cells in vitro. Infection and Immunity 35:79–85
    [Google Scholar]
  19. Segal E., Lehrer N., Ofek I. 1982; Adherence of Candida albicans to human vaginal epithelial cells: inhibition by amino sugars. Experimental Cell Biology 50:13–17
    [Google Scholar]
  20. Sharon N., Lis H. 1972; Lectins: cell-agglutinating and sugar-specific proteins. Science 111:949–959
    [Google Scholar]
  21. Sharon N., Eshdat Y., Silverblatt F.J., Ofek I. 1981; Bacterial adherence to cell surface sugars. Adhesion and Microorganism Pathogenicity Ciba Foundation Symposium no. 80 pp 119–141 O’Connor M., Whelan J., Elliott K. Edited by London: Pitman Medical;
    [Google Scholar]
  22. Sobel J.D., Myers P.G., Kaye D., Levison M.E. 1981; Adherence of Candida albicans to human vaginal and buccal epithelial cells. Journal of Infectious Diseases 143:76–82
    [Google Scholar]
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