1887

Abstract

Yeast cells of 1001 produced glucan-hydrolysing activity, most of which was due to an exo-1,3--glucanase. The enzyme was periplasmically located; it could be found in culture medium samples, and was secreted by protoplasts when cultured under regeneration conditions. In contrast to most yeast exoglucanases, this enzyme was practically inactive against -nitrophenyl---glucoside, hydrolysis of this substrate being carried out by a -glucosidase located inside the cytoplasmic membrane and not secreted to the external medium. Supernatant fluids from cell-free extracts reached their maximum glucanase level after several days at 0 °C, suggesting that the active enzyme was formed from an inactive precursor. Glucanase activity substantially decreased and sometimes disappeared from the cells when the yeast-to-mycelium transition was induced, but a significant (though lesser) reduction was also observed in yeast cells incubated in the same medium under conditions (temperature, cell concentration) that did not lead to formation of hyphae. It is suggested that exo-1,3--glucanase may not be necessary for mycelial growth.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-3-609
1987-03-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/3/mic-133-3-609.html?itemId=/content/journal/micro/10.1099/00221287-133-3-609&mimeType=html&fmt=ahah

References

  1. Ashwell G. 1957; Colorimetric analysis of sugars. Methods in Enzymology 3:73–105
    [Google Scholar]
  2. Barrett-Bee K.J., Lees J., Henderson W. 1982; Variation in the activities of enzymes associated with cell wall metabolism during a growth cycle of Candida albicans.. FEMS Microbiology Letters 15:275–278
    [Google Scholar]
  3. Brock T.D. 1965; β-Glucanase OF yeast. Biochemical and Biophysical Research Communications 19:623–629
    [Google Scholar]
  4. Chiew Y.Y., Shepherd M.G., Sullivan P.A. 1980; Regulation of chitin synthesis during germtube formation in Candida albicans.. Archives of Microbiology 125:97–104
    [Google Scholar]
  5. Fleet G.H. 1984; The occurrence and function of endogenous wall-degrading enzymes in yeasts. In Microbial Cell Wall Synthesis and Autolysis227–238 pp. Nombela C. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  6. Gale E.F. 1974; The release of potassium ions from Candida albicans in the presence of polyene antibiotics. Journal of General Microbiology 80:451–465
    [Google Scholar]
  7. Gopal P.K., Shepherd M.G., Sullivan P.A. 1984; Analysis of wall glucans from yeast, hyphal and germ tube forming cells of Candida albicans.. Journal of General Microbiology 130:3295–3301
    [Google Scholar]
  8. Hubbard M.J., Markie D., Poulter R.T.M. 1986; Isolation and morphological characterization of a mycelial mutant of Candida albicans. Journal of Bacteriology 165:61–65
    [Google Scholar]
  9. Johnson B. 1968; Lysis of yeast cell walls induced by 2-deoxyglucose at their sites of glucan synthesis. Journal of Bacteriology 95:1169–1172
    [Google Scholar]
  10. Jones D., Gordon A.H., Bacon J.S.D. 1974; Cooperative action by endo and exo-1,3-β-glucanase from parasitic fungi in the degradation of cell wall glucans of Sclerotinia sclerotiorum.. Biochemical Journal 140:47–55
    [Google Scholar]
  11. Keston A.S. 1956; Specific colorimetric reagents for glucose. Abstracts of the 129th Meeting of the American Chemical Society p. 31C:
    [Google Scholar]
  12. Lee K.L., Buckley H.R., Campbell C.C. 1975; An amino acid liquid synthetic medium for the development of mycelial and yeast forms of Candida albicans.. Sabouraudia 13:148–153
    [Google Scholar]
  13. Manning M., Mitchell T.G. 1980; Strain variation and mycelial phase Candida albicans in low-sulfate, synthetic medium. Journal of Bacteriology 142:714–719
    [Google Scholar]
  14. Niimi M., Niimi K., Tokunaga J., Nakayama H. 1980; Changes in cyclic nucleotide levels and dimorphic transitions in Candida albicans.. Journal of Bacteriology 142:1010–1014
    [Google Scholar]
  15. Notario V. 1982; β-Glucanases from Candida albicans : purification, characterization and the nature of their attachment to cell wall components. Journal of General Microbiology 128:747–759
    [Google Scholar]
  16. Odds F.C. 1985; Morphogenesis in Candida albicans. CRC Critical Reviews in Microbiology 12:45–80
    [Google Scholar]
  17. Phaff H.J. 1979; A retrospective and current view on endogenous β-glucanases in yeast. In Advances in Protoplast Research (Proceedings of the 5th International Protoplast Symposium) pp. 171–182 Budapest: Hungarian Academy of Sciences;
    [Google Scholar]
  18. Pomes R., Gil C., Nombela C. 1985; Genetic analysis of Candida albicans morphological mutants. Journal of General Microbiology 131:2107–2113
    [Google Scholar]
  19. Ram S.P., Romana L.K., Shepherd M.G., Sullivan P.A. 1984; Exo-l,3-β-glucanase, autolysin and trehalase activities during yeast growth and germ tube formation in Candida albicans. Journal of General Microbiology 130:1227–1236
    [Google Scholar]
  20. Santos T., Rey F., Conde J., Villanueva J.R., Nombela C. 1979; Saccharomyces cerevisiae mutant defective in exo-l,3-β-glucanase production. Journal of Bacteriology 139:333–338
    [Google Scholar]
  21. Shepherd M.G., Chiew Y.Y., Ram S.P., Sullivan P.A. 1980; Germ tube induction in Candida albicans.. Canadian Journal of Microbiology 26:21–26
    [Google Scholar]
  22. Somogyi M. 1952; Notes on sugar determination. Journal of Biological Chemistry 195:19–23
    [Google Scholar]
  23. Sullivan P.A., Chiew Y.Y., Molloy C., Templeton M.D., Shepherd M.G. 1983; An analysis of the metabolism and cell wall composition of Candida albicans during germ-tube formation. Canadian Journal of Microbiology 29:1514–1525
    [Google Scholar]
  24. Tomasz A., Westphal M. 1971; Abnormal autolytic enzyme in a pneumococcus with altered teichoic acid composition. Proceedings of the National Academy of Sciences of the United States of America 68:2627–2630
    [Google Scholar]
  25. Villanueva J.R., Gacto M., Duran A. 1979; Endogenous lytic enzymes and the yeast cell wall. In Advances in Protoplast Research (Proceedings of the 5th International Protoplast Symposium) pp. 183–197 Budapest: Hungarian Academy of Sciences;
    [Google Scholar]
  26. Whelan W.L., Soll D.R. 1982; Mitotic recombination in Candida albicans : recessive lethal alleles linked to a gene required for methionine biosynthesis. Molecular and General Genetics 180:107–113
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-3-609
Loading
/content/journal/micro/10.1099/00221287-133-3-609
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error