SUMMARY: The binding of human fibrinogen to germ-tubes and mycelium of , forms usually found in infected tissues, was studied by an immunofluorescence assay. Binding was quantified by using I-labelled fibrinogen. The degree of binding differed according to the morphological form of the fungus. Binding relative to that of the yeast form was greater for mycelium (12-fold) than for germ-tube (7·7-fold). Pretreatment of yeasts with fragments D and E (terminal degradation products of fibrinogen) before fibrinogen binding showed that fragment D possessed a higher affinity for than fragment E. Binding of fibrinogen was diminished when was pretreated with 2-mercaptoethanol alone or in combination with pronase, or pretreated with α-mannosidase or trypsin. Binding was not decreased by pretreatment with pronase alone or chitinase. Inhibition experiments using dialysed culture filtrate, mannan, chitin, sugars or amino sugars were done by preabsorbing the fibrinogen with each of the above mentioned compounds. dialysed culture filtrate inhibited the binding more strongly than mannan. However, fibrinogen binding to was not significantly reduced by mannose, several other sugars or chitin. These studies demonstrate the existence of a fibrinogen-binding factor (FBF) strongly associated with the surface of germ-tube and filamentous forms of , and indicate a possible role for FBF in the pathogenicity of .


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