Cells of a strain of a Citrobacter sp., pre-grown in cadmium-free continuous culture, accumulated cadmium extensively when resuspended in a buffer that contained Cd2+ and glycerol 2-phosphate. The accumulated compound was identified by X-ray microanalysis and magic angle spinning NMR analysis as cell-bound cadmium phosphate, probably CdHPO4. Its accumulation is consistent with the activity of a phosphatase, induced during pre-growth, that continues to function in the resuspended cells to liberate HPO2−4 from glycerol 2-phosphate. This anion then combines with Cd2+ to form insoluble cell-bound CdHPO4.
AickinR. M.,
DeanA. C. R.,
CheethamA. K.,
SkarnulisA. J.1979; Electron microscope studies on the uptake of lead by a Citrobacter sp. Microbios Letters 9:7–15
CheethamA. K.,
ClaydenN. J.,
DobsonC. M.,
JakemanR. J. B.1986; Correlations between 3IPN.M.R.chemical shifts and structural parameters in crystalline inorganic phosphates. Journal of the Chemical Society195–197
KawabeH.,
SugiuraY.,
TanakeH.1981; 31P and 17O NMR of Mn(III)-containing acid phosphatase. Biochemical and Biophysical Research Communications 103:327–331
MacaskieL. E.,
WatesJ. M.,
DeanA. C. R.1987; Cadmium accumulation by a Citrobacter sp. immobilized on gel and solid supports: applicability to the treatment of wastes containing heavy metal cations. Biotechnology and Bioengineering (in the Press)
MichelL. J.,
MacaskieL. E.,
DeanA. C. R.1986; Cadmium accumulation by immobilized cells of a Citrobacter sp. using various phosphate donors. Biotechnology and Bioengineering 28:1358–1365
WetzelB. K.,
SpicerS. S.,
DvorakH. F.,
HeppelL. A.1970; Cytochemical localization of certain phosphatases in Escherichia coli. Journal of Bacteriology 104:529–542