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SUMMARY: In glucose-grown cells of Saccharomyces cerevisiae, collected during the stationary phase of growth, the addition of K+ to the external medium reversed glucose-induced internal acidification in 2 min. However, in ethanol-grown cells external K+ did not reverse the effect of glucose even after 20 min. The presence or absence of external K+ did not alter the modification of trehalase and fructose-1,6-bisphosphatase induced by glucose. It is concluded that transient acidification may be sufficient to cause the associated transient increase in cAMP.
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