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SUMMARY: In preparation of Bacillus thuringiensis protoplasts or cell-free lysates, hydrolysis of cell walls was due to endogenous autolytic activity and was largely independent of lysozyme. Purified cell walls of B. thuringiensis were resistant to lysozyme, lysostaphin, chitinase, trypsin and Bacillus subtilis proteinase, but were hydrolysed by B. thuringiensis autolytic enzymes or an N-acetylmuramidase (mutanolysin) from Streptomyces globisporus. Mutanolysin was effective in preparation of protoplasts or lysates of B. thuringiensis, and, unlike lysozyme, did not interfere with electrophoretic analysis of DNA.
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