1887

Abstract

SUMMARY: In preparation of protoplasts or cell-free lysates, hydrolysis of cell walls was due to endogenous autolytic activity and was largely independent of lysozyme. Purified cell walls of were resistant to lysozyme, lysostaphin, chitinase, trypsin and proteinase, but were hydrolysed by autolytic enzymes or an -acetylmuramidase (mutanolysin) from . Mutanolysin was effective in preparation of protoplasts or lysates of , and, unlike lysozyme, did not interfere with electrophoretic analysis of DNA.

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/content/journal/micro/10.1099/00221287-133-3-503
1987-03-01
2022-01-19
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