1887

Abstract

A series of improved phage vectors have been constructed, based on bacteriophage ø105, which can be used to clone genes in by direct transfection of protoplasts. The new vectors, designated ø105J23, ø105J24, ø105J27 and ø105J28, show frequencies of plaque formation that are equal to those of wild-type ø105. This represents at least a 10-fold improvement over ø105J9, the vector used in previous cloning experiments. Two of the new vectors ø105J27 and ø105J28 incorporate a mutation, , that renders the prophage temperature inducible. This has made it possible to devise a rapid small-scale procedure for screening progeny phage for the presence of inserted DNA. The usefulness of the new vectors is illustrated in the accompanying paper by cloning more than 20 sporulation genes.

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1987-03-01
2021-07-31
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