1887

Abstract

Escherichia coli K12 grows on 2-phenylethylamine as sole carbon and energy source by converting it, via phenylacetaldehyde, to phenylacetic acid. Phenylacetaldehyde was formed by the action of an inducible amine oxidase and catalase activity was increased sixfold, presumably to ensure removal of the H202 that was expected to be a product of the amine oxidation. The phenylacetaldehyde was oxidized to phenylacetic acid by an inducible NAD+-dependent dehydrogenase. Mutants defective in phenylacetaldehyde dehydrogenase cannot grow on 2-phenylethylamine as carbon and energy source but can still use it as a nitrogen source.

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/content/journal/micro/10.1099/00221287-133-2-347
1987-02-01
2021-10-20
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References

  1. Bachmann B. J. 1972; Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriological Reviews 36:525–557
    [Google Scholar]
  2. Beers R. F., Sizer I. W. 1952; A spectrophoto-metric method for measuring the breakdown of hydrogen peroxide by catalase. Journal of Biological Chemistry 195:133–140
    [Google Scholar]
  3. Blaschko H. 1963; Amine oxidase. In The Enzymes,, 2nd edn.. 8 pp 337–551 Boyer P. D., Lardy H., Myrbäck K. Edited by New York & London: Academic Press;
    [Google Scholar]
  4. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Burlingame R., Chapman P. J. 1983; Catabolism of phenylpropionic acid and its 3-hydroxy derivative by Escherichia coli. . Journal of Bacteriology 155:113–121
    [Google Scholar]
  6. Cooper R. A., Skinner M. A. 1980; Catabolism of 2-and 4-hydroxyphenylacetate by the 3,4-dihydroxy- phenylacetate pathway in Escherichia coli. . Journal of Bacteriology 143:302–306
    [Google Scholar]
  7. Cooper R. A., Jones D. C. N., Parrott S. 1985; Isolation and mapping of Escherichia coli K12 mutants defective in phenylacetate degradation. Journal of General Microbiology 131:2753–2757
    [Google Scholar]
  8. Donnelly M. I., Cooper R. A. 1981; Succinic semialdehyde dehydrogenases of Escherichia coli: their role in the degradation of p-hydroxyphenyl- acetate and γ-aminobutyrate. European Journal of Biochemistry 113:555–561
    [Google Scholar]
  9. Duine J. A., FrankJzn J., Jongejan J. A. 1986; PQQ and quinoprotein enzymes in microbial oxidations. FEMS Microbiology Reviews 32:165–178
    [Google Scholar]
  10. Fujioka M., Morino Y., Wada H. 1970; Metabolism of phenylalanine. (Achromobacter eurydice). Methods in Enzymology 17a:585–596
    [Google Scholar]
  11. Hassan H. M., Fridovich I. 1978; Regulation of the synthesis of catalase and peroxidase in Escherichia coli . Journal of Biological Chemistry 253:6445–6450
    [Google Scholar]
  12. Haywood G. W., Large P. J. 1981; Microbial oxidation of amines. Distribution, purification and properties of two primary-amine oxidases from the yeast Candida boidinii grown on amines as sole nitrogen source. Biochemical Journal 199:187–201
    [Google Scholar]
  13. Levering P. R., vanDijken J. P., Veenhuis M., Harder W. 1981; Arthrobacter P1, a fast growing versatile methylotroph with amine oxidase as a key enzyme in the metabolism of methylated amines. Archives of Microbiology 129:72–80
    [Google Scholar]
  14. Loewen P. C. 1984; Isolation of catalase-deficient Escherichia coli mutants and genetic mapping of katE, a locus that affects catalase activity. Journal of Bacteriology 157:622–626
    [Google Scholar]
  15. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  16. Scarlett F. A., Turner J. M. 1976; Microbial metabolism of amino alcohols. Ethanolamine catabolism mediated by coenzyme B12-dependent ethanolamine ammonia-lyase in Escherichia coli and Klebsiella aerogenes . Journal of General Microbiology 95:173–176
    [Google Scholar]
  17. Yoshpe-Purer Y., Henis Y., Yasphe J. 1977; Regulation of catalase level in Escherichia coli K12. Canadian Journal of Microbiology 23:84–91
    [Google Scholar]
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