Change of -Ascorbic Acid Content in Synchronized Cultures of Free

Abstract

The content of total -ascorbic acid in light-dark synchronized Z increased rapidly with illumination to reach a maximum after 7 h in the light and then decreased to reach its original level after 18 h in the dark. Total -ascorbic acid formation was strongly dependent on illumination and was inhibited by cycloheximide, but not by chloramphenicol or streptomycin. Inhibitors of respiration and photosynthesis markedly inhibited -ascorbic acid formation, indicating that the change of the -ascorbic acid content may be related to the metabolic activities of mitochondria and chloroplasts.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-2-221
1987-02-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/2/mic-133-2-221.html?itemId=/content/journal/micro/10.1099/00221287-133-2-221&mimeType=html&fmt=ahah

References

  1. Bovarnick J. G., Chang S. W., Schiff J. A., Schwartbach S. D. 1974a; Events surrounding the early development of Euglena chloroplasts: experiments with streptomycin in non-dividing cells. Journal of General Microbiology 83:51–62
    [Google Scholar]
  2. Bovarnick J. G., Schiff J. A., Freedman Z., Egan J. M. Jr 1974b; Events surrounding the early development of Euglena chloroplasts: cellular origins of chloroplast enzymes in Euglena . Journal of General Microbiology 83:63–71
    [Google Scholar]
  3. Cook J. R. 1971; Synchronous cultures: Euglena . Methods in Enzymology 23:74–78
    [Google Scholar]
  4. Cook J. R., Hess M. 1964; Sulfur-containing nucleotides associated with cell division in synchronized Euglena gracilis . Biochimica et biophysica acta 80:148–151
    [Google Scholar]
  5. Cook J. R., James T. W. 1960; Light-induced division synchrony in Euglena gracilis var. bacillaris . Experimental Cell Research 21:583–589
    [Google Scholar]
  6. Cramer M., Myers J. 1952; Growth and photosynthetic characteristics of Euglena gracilis . Archiv fur Mikrobiologie 17:384–402
    [Google Scholar]
  7. Edmunds L. N. Jr 1964; Replication of DNA and cell division in synchronously dividing cultures of Euglena gracilis . Science 145:266–268
    [Google Scholar]
  8. Gerhardt B. 1964; The relation of ascorbic acid to photosynthesis. Planta 61:101–129
    [Google Scholar]
  9. Osafune T., Mihara S., Hase E., Ohkuro I. 1975; Formation and division of giant mitochondria during the cell cycle of Euglena gracilis Z in synchronous culture. I. Some characteristics of changes in the morphology of mitochondria and oxygen-uptake activity of cells. Plant and Cell Physiology 16:313–326
    [Google Scholar]
  10. Shigeoka S., Yokota A., Nakano Y., Kitaoka S. 1979a; The effect of illumination on the l-ascorbic acid content in Euglena gracilis Z. Agricultural and Biological Chemistry 43:2053–2058
    [Google Scholar]
  11. Shigeoka S., Nakano Y., Kitaoka S. 1979b; The biosynthetic pathway of l-ascorbic acid in Euglena gracilis Z. Journal of Nutritional Science and Vitaminology 25:299–307
    [Google Scholar]
  12. Shigeoka S., Nakano Y., Kitaoka S. 1980; Metabolism of hydrogen peroxide in Euglena gracilis Z by l-ascorbic acid. Biochemical Journal 186:377–380
    [Google Scholar]
  13. Walther W. G., Edmunds L. N. Jr 1973; Studies on the control of the rhythm of photosynthetic capacity in synchronized cultures of Euglena gracilis Z. Plant Physiology 51:250–258
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-2-221
Loading
/content/journal/micro/10.1099/00221287-133-2-221
Loading

Data & Media loading...

Most cited Most Cited RSS feed