The content of total L-ascorbic acid in light-dark synchronized Z increased rapidly with illumination to reach a maximum after 7 h in the light and then decreased to reach its original level after 18 h in the dark. Total L-ascorbic acid formation was strongly dependent on illumination and was inhibited by cycloheximide, but not by chloramphenicol or streptomycin. Inhibitors of respiration and photosynthesis markedly inhibited L-ascorbic acid formation, indicating that the change of the L-ascorbic acid content may be related to the metabolic activities of mitochondria and chloroplasts.


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