SUMMARY: The existence of energy-dependent Mn influx in ATCC 11414 was demonstrated both with mycelia grown for production of citric acid and in resting mycelia suspended in ‘Good’-buffers. A specific high-affinity transport system was detected at submicromolar concentrations of Mn which functioned independently of the transport of Mg and Ca, but was preferentially inhibited by Zn, Cu, Cd. The rate of Mn transport was suboptimal in the citric acid production medium (pH 3.8 or lower) as the pH optimum was found to be 5.6 in the buffer experiments.


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