SUMMARY: The gene of encodes a transcriptional activator (FNR) which is required for the expression of a number of genes involved in anaerobic respiratory pathways. From the study of a translational fusion of to the gene for β-galactosidase () it has been concluded that the gene is expressed under both aerobic and anaerobic conditions and is subject to autoregulation and repression by glucose, particularly during anaerobic growth. These findings imply that during anaerobiosis the FNR protein adopts an active conformation, in which it functions both as a repressor of the gene and as an activator of -dependent genes. Sequences in the 5’ non-coding region of which could be involved in autoregulation are discussed. The coding region was cloned into an expression vector which has allowed an amplification of FNR synthesis such that it accounts for about 2% of total cell protein. The ability to over-produce FNR in this way should be very useful for future biochemical studies.


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