SUMMARY: H is lysogenic for a large temperate phage we call H2. H2 has a polyhedral head 85 nm in diameter and a tail of about 17 × 434 nm. H2 between the and genes, and gives specialized transduction of and, at lower frequencies, of and . The phage carries a thymidylate synthase gene and converts thymine auxotrophs of to prototrophy. The H2 genome is a linear DNA molecule about 129 kb in length. DNA extracted from phage particles grown in is not cut by the restriction endonucleases , , l2861, and ; the latter enzyme is produced by strain H. The prophage in lysogenic cells can be cut by these enzymes. We have isolated H2 mutants that carry the transposon Tn917, or a mutation resulting in clear-plaque morphology, or both.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error