SUMMARY: Using a gene probe derived from the cloned var. insecticidal crystal protein (ICP) gene, we have cloned a var. HD-133 ICP gene in . The gene encodes a polypeptide that is toxic to Lepidoptera and . The protein is expressed at a level sufficient to produce phase-bright inclusions in recombinant strains, and these inclusions can be partially purified using discontinuous sucrose density gradients. Immunoblot- ting shows that the inclusions contain a 135 kDa polypeptide which reacts strongly with antiserum raised against the var. HD-I P1 polypeptide.


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