1887

Abstract

SUMMARY: A 19 kb I DNA fragment containing the gene for the extracellular active-site serine β-lactamase of KCC-SO352 was cloned in TK24 using the high-copy-number plasmid pIJ702 as vector. A 30-fold higher yield of β-lactamase was obtained from strain ML1, carrying the recombinant plasmid pDML51, than from grown under optimal production conditions. In all respects (molecular mass, isoelectric point, kinetics of inhibition by β-iodopenicillanate) the overproduced ML 1 β-lactamase was identical to the original enzyme. A considerable reduction of β-lactamase production was caused by elimination of a 12.8 kb portion of the 19 kb DNA fragment by cleavage at an internal I site located more than 3 kb upstream of the β-lactamase structural gene. The β-lactamase gene was located within a 1.8 NcoI-BclI fragment but when this fragment was cloned in pIJ702, the resulting strain produced hardly any more β-lactamase than the original .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-10-2915
1987-10-01
2019-11-20
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-10-2915
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error