%0 Journal Article %A Angus, Barbara L. %A Fyfe, Janet A. M. %A Hancock, Robert E. W. %T Mapping and Characterization of Two Mutations to Antibiotic Supersusceptibility in Pseudomonas aeruginosa %D 1987 %J Microbiology, %V 133 %N 10 %P 2905-2914 %@ 1465-2080 %R https://doi.org/10.1099/00221287-133-10-2905 %I Microbiology Society, %X SUMMARY: Two mutations associated with antibiotic supersusceptibility in Pseudomonas aeruginosa strain Z61 were transferred separately into strain PA0222, using R68.45-mediated conjugation and phage F116L transduction. One mutation (absA) was 40% contransducible with pro-82 at 26 min on the P. aeruginosu chromosome and was associated with increased susceptibility to β-lactams, gentamicin and hydrophobic agents. Strains carrying the absA mutation also displayed enhanced uptake of a hydrophobic fluorescent probe, 1-N-phenylnaphthylamine, and were found, by SDS-PAGE, to be altered in the pattern of lipopolysaccharide O-antigen distribution. The other mutation (absB), associated with increased susceptibility to β-lactams and gentamicin but not to hydrophobic agents, was cotransducible with met-28 and proC at 20 min on the chromosome. The absB mutation caused a structurally undefined alteration in the physical interaction of EDTA and gentamicin with the outer membrane. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-133-10-2905