1887

Abstract

SUMMARY: Two mutations associated with antibiotic supersusceptibility in strain Z61 were transferred separately into strain PA0222, using R68.45-mediated conjugation and phage F116L transduction. One mutation (A) was 40% contransducible with -82 at 26 min on the chromosome and was associated with increased susceptibility to β-lactams, gentamicin and hydrophobic agents. Strains carrying the A mutation also displayed enhanced uptake of a hydrophobic fluorescent probe, 1--phenylnaphthylamine, and were found, by SDS-PAGE, to be altered in the pattern of lipopolysaccharide O-antigen distribution. The other mutation (B), associated with increased susceptibility to β-lactams and gentamicin but not to hydrophobic agents, was cotransducible with -28 and C at 20 min on the chromosome. The B mutation caused a structurally undefined alteration in the physical interaction of EDTA and gentamicin with the outer membrane.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-10-2905
1987-10-01
2019-10-23
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-10-2905
Loading
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error