1887

Abstract

pv. (ISPP List 1980) and pv. (ISPP List 1980) strains, isolated from cassava () plants of different geographical origin, were studied by numerical analysis of 267 phenotypic features, computer-assisted comparison of gel electrophoregrams of soluble proteins, mol% G + C determinations, DNA: DNA hybridizations and virulence tests. pv. and pv. constituted separate biological entities which could be differentiated from each other by four biochemical features, their symptoms on cassava, their soluble protein electrophoregrams and their DNA characteristics. Within each pathovar no correlation was found between phytopathogenicity, geographic origin and year of isolation of the strains, on the one hand, and the biochemical, physiological and protein electrophoretic properties on the other. Two yellowish strains, CIAT 1164 and CIAT 1165, isolated from cassava in Colombia were genetically and electrophoretically similar to pv. , but were unable to infect pv. was genetically more related to the pv. cluster than to the pv. cluster.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-1-57
1987-01-01
2024-12-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/1/mic-133-1-57.html?itemId=/content/journal/micro/10.1099/00221287-133-1-57&mimeType=html&fmt=ahah

References

  1. Barritt M. M. 1936; The intensification of Voges- Proskauer reaction by the addition of β-naphthol. Journal of Pathological Bacteriology 42:441–454
    [Google Scholar]
  2. Bauer A. W., Kirby W. M. M., Sherris J. C., Turck M. 1966; Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology 45:493–496
    [Google Scholar]
  3. Bonham-Carter G. F. 1967 Fortran IV Program for Q-mode Cluster Analysis of Nonquantitative Data using IBM 7090/7094 Computers. Kansas Geological Survey Computer Program Lawrence, USA: The University of Kansas;
    [Google Scholar]
  4. Bradbury J. F. 1984; Genus II. Xanthomonas Dowson 1939, 187. In Bergey’s Manual of Systematic Bacteriology pp. 199–210 Krieg N. R. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  5. De Ley J. 1970; Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. Journal of Bacteriology 101:738–754
    [Google Scholar]
  6. De Ley J., Van Muylem J. 1963; Some applications of deoxyribonucleic acid base composition in bacterial taxonomy. Antonie van Leeuwenhoek 29:344–358
    [Google Scholar]
  7. De Ley J., Cattoir H., Reynaerts A. 1970; The quantitative measurement of DNA hybridization from renaturation rates. European Journal of Biochemistry 12:133–142
    [Google Scholar]
  8. De Vos P., De Ley J. 1983; Intra-and intergeneric similarities of Pseudomonas and Xanthomonas ribosomal ribonucleic acid cistrons. International Journal of Systematic Bacteriology 33:487–509
    [Google Scholar]
  9. Doetsch R. N. 1981; Determinative methods of light microscopy. In Manual of Methods for General Bacteriology pp. 21–33 Gerhardt P., Murray R. G. E., Costilow R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  10. Dos Santos R.M.D.B., Dianese J. C. 1985; Comparative membrane characterization of Xanthomonas campestris pv. cassavae and X. campestris pv. manihotis. Phytopathology 75:581–587
    [Google Scholar]
  11. Dowson W. J. 1939; On the systematic position and generic names of the Gram negative bacterial plant pathogens. Zentralblatt für Bakteriologie, Parasiten- kunde, Infektionskrankheiten und Hygiene (Abteilung II) 100:177–193
    [Google Scholar]
  12. Dye D. W. 1962; The inadequacy of the usual determinative tests for the identification of Xanthomonas spp. New Zealand Journal of Science 5:393–416
    [Google Scholar]
  13. Dye D. W., Bradbury J. F., Goto M., Hayward A. C., Lelliott R. A., Schroth M. N. 1980; International standards for naming pathovars of phytopathogenic bacteria and a list of pathovar names and pathotype strains. Review of Plant Pathology 59:153–168
    [Google Scholar]
  14. Elango F. N., Lozano J. C., Peterson J. F. 1981; Relationships between Xanthomonas c. pv. manihotis, X. c. pv. cassavae and Colombian yellowish isolates. Proceedings of the Fifth International Conference on Plant Pathogenic Bacteria pp. 96–104 Cali, Colombia: CIAT;
    [Google Scholar]
  15. Kersters K. 1985; Numerical methods in the classification of bacteria by protein electrophoresis. In Computer-assisted Bacterial Systematics (Special Publication of the Society for General Microbiology) pp. 337–368 Goodfellow M., Jones D., Priest F. G. Edited by London: Academic Press;
    [Google Scholar]
  16. Kersters K., De Ley J. 1975; Identification and grouping of bacteria by numerical analysis of their electrophoretic protein patterns. Journal of General Microbiology 87:333–342
    [Google Scholar]
  17. Kimura O., Dianese J. C. 1983; Caracterizacão proteica e isoenzimática dos patovares de X. campestris (Pammel) Dowson que atacam a mandioca. Pesquisa Agropecuaria Brasileira 18:1215–1228
    [Google Scholar]
  18. Kovacs N. 1928; Eine vereinfachte Methode zum Nachweis der Indolbildung durch Bakterien. Zeitschrift für Immunitätsforschung und experimentelle Therapie 55:311–315
    [Google Scholar]
  19. Kovacs N. 1956; Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature; London: 178703
    [Google Scholar]
  20. Maraite H., Perreaux D. 1979; Comparative symptom development in cassava after infection by Xanthomonas manihotis or X. cassavae under controlled conditions. In Cassava Bacterial Blight in Africa.Past, Present and Future pp. 17–24 Terry E., Persley G., Cook S. Edited by London: COPR;
    [Google Scholar]
  21. Maraite H., Weyns J. 1979; Distinctive physiological, biochemical and pathogenic characteristics of Xanthomonas manihotis and X. cassavae. In Diseases of Tropical Food Crops pp. 103–117 Maraite H., Meyer J. A. Edited by Louvain-la-Neuve, Belgium: Université Catholique de Louvain;
    [Google Scholar]
  22. Maraite H., Weyns J., Yimkwan O., Lipemba P., Perreaux D. 1981; Physiological and pathogenic variations in Xanthomonas campestris pv. manihotis. Proceedings of the Fifth International Conference on Plant Pathogenic Bacteria pp. 358–368 Cali, Colombia: CIAT;
    [Google Scholar]
  23. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  24. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  25. Pammel L. H. 1895; Bacteriosis of rutabaga, (Bacillus campestris n. sp.). Iowa State College Agricultural Experimental Station Bulletin 27:130–134
    [Google Scholar]
  26. Paton A. M. 1959; An improved method for preparing pectate gels. Nature; London: 1831812–1813
    [Google Scholar]
  27. Robbs C. F., Ribeiro R., Kimura O., Akiba F. 1972; Variações en Xanthomonas manihotis (Arthaud-Berthet) Starr. Revista da Sociedade Brasileira de Fitopatologia 5:67–75
    [Google Scholar]
  28. Sabet K. A., Ishag F., Khalil O. 1969; Studies on the bacterial diseases of Sudan crops. VII. New records. Annals of Applied Biology 63:357–369
    [Google Scholar]
  29. Schaad N. W. editor 1980 Laboratory Guide for Identification of Plant Pathogenic Bacteria St Paul, Minn., USA: Bacteriological Committee of American Phytopathological Society;
    [Google Scholar]
  30. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy. The Principles and Practice of Numerical Classification San Francisco: W. H. Freeman;
    [Google Scholar]
  31. Sokal R. R., Michener C. D. 1958; A statistical method for evaluating systematic relationships. University of Kansas Scientific Bulletin 38:1409–1438
    [Google Scholar]
  32. Starr M. P. 1945; The nutrition of phytopathogenic bacteria. I. Minimal nutritive requirements of the genus Xanthomonas. Journal of Bacteriology 51:131–143
    [Google Scholar]
  33. Swings J., Kersters K., De Ley J. 1976; Numerical analysis of electrophoretic protein patterns of Zymomonas strains. Journal of General Microbiology 93:266–271
    [Google Scholar]
  34. Van den Mooter M., Swings J., De Cleene M., Leyns F., De Ley J. 1981; Sensitivity of Xanthomonas towards antibiotics in vitro. Mededelingen van de Faculteir Landbouwwetenschappen Rijksuniversiteit Gent 46:787–792
    [Google Scholar]
  35. Van den Mooter M. 1984 De taxonomie van het plantpathogene bakteriengeslacht Xanthomonas Dowson 1939 PhD thesis Rijksuniversiteit Gent, Belgium:
    [Google Scholar]
  36. Van den Mooter M., Steenackers M., Maertens C., Gossele F., De Vos P., Swings J., Kersters K., De Ley J. 1986; Differentiation between Xanthomonas campestris pv. graminis (ISPP List 1980), pv. phleipratensis (ISPP List 1980) emend., pv. poae Egli and Schmidt 1982 and pv. arrhenatheri Egli and Schmidt 1982, by numerical analysis of phenotypic features and protein gel electrophoregrams. Phytopathologische Zeitschrift in the Press
    [Google Scholar]
  37. Vera Cruz C. M., Gossele F., Kersters K., Segers P., Van den Mooter M., Swings J., De Ley J. 1984; Differentiation between Xanthomonas campestris pv. oryiae, Xanthomonas campestris pv. oryzicola and the bacterial ‘brown blotch’ pathogen on rice by numerical analysis of phenotypic features and protein gel electrophoregrams. Journal of General Microbiology 130:2983–2999
    [Google Scholar]
  38. Wishart D. 1978 Clustan User Manual, Version IC, Release 2, 3rd edn. Edinburgh: Program Library Unit, Edinburgh University;
    [Google Scholar]
/content/journal/micro/10.1099/00221287-133-1-57
Loading
/content/journal/micro/10.1099/00221287-133-1-57
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error