1887

Abstract

A total of 564 strains of heterotrophic non-halophilic, marine, moderately halophilic and extremely halophilic bacteria was isolated from a saltern located near Huelva, SW Spain, facing the Atlantic Ocean. With the exception of the extremely halophilic bacteria, these were analysed by numerical techniques using the simple matching () coefficient and clustering was achieved using the unweighted pair group method with averages (UPGMA) algorithm. At the 72.5% similarity level, a different number of phenons was found for each salinity group of bacteria. Of the 154 non-halophilic strains, the majority were recovered in 10 phenons, representing members of the genera and The 138 marine strains, with the exception of 24 strains, clustered into 12 phenons; seven phenons were assigned to the suprageneric grouping , and the other phenons to and The 140 moderately halophilic strains grouped into eight phenons, with only 11 strains left unassigned. The majority of these isolates were assigned to the genus (two phenons) and they showed great similarity with the recently described species , isolated from hypersaline soils. Four phenons comprised microorganisms with phenotypic characteristics very similar to the moderately halophilic Two phenons were assigned to the genera and but these strains were not similar to previously described species. Of the 145 extremely halophilic isolates studied, the most important group was identified as belonging to the species (86 strains), followed by (20 strains), (19 strains), (12 strains) and (7 strains). Only one coccus was isolated, with characteristics very similar to those of

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1987-01-01
2021-08-05
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References

  1. Baumann P., Schubert R. H. W. 1984; Family Vibrionaceae. In Bergey’s Manual of Systematic Bacteriology 1 pp. 516–538 Krieg N. R. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Baumann P., Furniss A. L., Lee J. V. 1984; Genus Vibrio. In Bergey’s Manual of Systematic Bacteriology 1 pp. 518–538 Krieg N. R. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  3. Bovre K. 1984; Family Neisseriaceae. In Bergey’s Manual of Systematic Bacteriology 1 pp. 288–309 Krieg N. R. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  4. Brisou J. F. editor 1980 Les Bacteries Marines Paris: Masson;
    [Google Scholar]
  5. Brisou J., Courtois D., Fenis F. 1974; Microbiological study of a hypersaline lake in French Somaliland. Applied Microbiology 27:819–822
    [Google Scholar]
  6. Buchanan R. E., Gibbons N. E. editor 1974 Bergey’s Manual of Determinative Bacteriology, 8th edn. Baltimore: Williams & Wilkins;
    [Google Scholar]
  7. Colwell R. R. editor 1984 Vibrios in the Environment New York: Wiley;
    [Google Scholar]
  8. Davis J. S. 1978; Biological communities of a nutrient enriched salina. Aquatic Botany 4:23–42
    [Google Scholar]
  9. Elazari-Volcani B. 1940 Studies on the microflora of the Dead Sea PhD thesis Hebrew University, Jerusalem:
    [Google Scholar]
  10. Forsyth M. P., Shindler D. B., Gochnauer M. B., Kushner D. J. 1971; Salt tolerance of intertidal marine bacteria. Canadian Journal of Microbiology 17:825–828
    [Google Scholar]
  11. Gibbons N. E. 1969; Isolation, growth and requirements of halophilic bacteria. Methods in Microbiology 3B:169–183
    [Google Scholar]
  12. Gunn B. A., Colwell R. R. 1983; Numerical taxonomy of staphylococci isolated from the marine environment. International Journal of Systematic Bacteriology 33:751–759
    [Google Scholar]
  13. Henis Y., Eren J. 1963; Preliminary studies on the microflora of a highly saline soil. Canadian Journal of Microbiology 9:902–904
    [Google Scholar]
  14. Holmes B., Owen R. J., Mcmeekin T. A. 1984; Genus Flavobacterium. In Bergey’s Manual of Systematic Bacteriology 1 pp. 353–360 Krieg N. R. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  15. Jaccard P. 1908; Nouvelles recherches sur la distribution florale. Bulletin de la Société vaudoise des sciences naturelles 44:223–270
    [Google Scholar]
  16. Javor B. J. 1984; Growth potential of halophilic bacteria isolated from solar salt environments: carbon sources and salt requirements. Applied and Environmental Microbiology 48:352–360
    [Google Scholar]
  17. Juez G., Rodriguez-Valera F., Ventosa A., Kushner D. J. 1986; Haloarcula hispanica spec, nov. and Haloferax gibbonsii spec, nov., two new species of extremely halophilic Archaebacteria. Systematic and Applied Microbiology 8:75–79
    [Google Scholar]
  18. Kushner D. J. 1978; Life in high salt and solute concentrations: halophilic bacteria. In Microbial Life in Extreme Environments pp. 317–368 Kushner D. J. Edited by London: Academic Press;
    [Google Scholar]
  19. Kushner D. J. 1985; The Halobacteriaceae. In The Bacteria 8 pp. 171–214 Woese C. R., Wolfe R. S. Edited by London: Academic Press;
    [Google Scholar]
  20. Larsen H. 1962; Halophilism. In The Bacteria 4 pp. 297–336 Gunsalus I. C., Stanier R. Y. Edited by London: Academic Press;
    [Google Scholar]
  21. Larsen H. 1984; Family Halobacteriaceae. In Bergey’s Manual of Systematic Bacteriology 1 pp. 261–267 Krieg N. R. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  22. Mullakhanbhai M. F., Larsen H. 1975; Halobacterium volcanii spec, nov., a Dead Sea Halobacter- ium with a moderate salt requirement. Archives of Microbiology 104:207–214
    [Google Scholar]
  23. Nissenbaum A. 1975; The microbiology and biogeochemistry of the Dead Sea. Microbial Ecology 2:139–161
    [Google Scholar]
  24. Oren A. 1983a; Halobacterium sodomense spec, nov., a Dead Sea Halobacterium with an extremely high magnesium requirement. International Journal of Systematic Bacteriology 33:381–386
    [Google Scholar]
  25. Oren A. 1983b; Clostridium lortetii spec, nov., a halophilic obligatory anaerobic bacterium producing endospores with attached gas vacuoles. Archives of Microbiology 136:42–48
    [Google Scholar]
  26. Oren A., Paster B. J., Woese C. R. 1984a; Haloanaerobiaceae: a new family of moderately halophilic obligatory anaerobic bacteria. Systematic and Applied Microbiology 5:71–80
    [Google Scholar]
  27. Oren A., Weisburg W. G., Kessel H., Woese C. R. 1984b; Halobacteroides halobius gen. nov., spec, nov., a moderately halophilic anaerobic bacterium from the bottom sediments of the Dead Sea. Systematic and Applied Microbiology 5:58–70
    [Google Scholar]
  28. Post F. J. 1977; The microbial ecology of the Great Salt Lake. Microbial Ecology 2:139–161
    [Google Scholar]
  29. Quesada E., Ventosa A., Rodriguez-Valera F., Megias L., Ramos-Cormenzana A. 1983; Numerical taxonomy of moderately halophilic Gram-negative bacteria from hypersaline soils. Journal of General Microbiology 129:2649–2657
    [Google Scholar]
  30. Quesada E., Ventosa A., Ruiz-Berraquero F., Ramos-Cormenzana A. 1984; Deleya halophila, a new species of moderately halophilic bacteria. International Journal of Systematic Bacteriology 34:287–292
    [Google Scholar]
  31. Quesada E., Bejar V., Valderrama M. J., Ventosa A., Ramos-Cormenzana A. 1985; Isolation and characterization of moderately halophilic nonmotile rods from different saline habitats. Microbiologia 1:89–96
    [Google Scholar]
  32. Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1979; Isolation of extreme halophiles from seawater. Applied and Environmental Microbiology 38:164–165
    [Google Scholar]
  33. Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1981; Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentrations. Microbial Ecology 7:235–243
    [Google Scholar]
  34. Rodriguez-Valera F., Juez G., Kushner D. J. 1983; Halobacterium mediterranei spec, nov., a new carbohydrate-utilizing extreme halophile. Systematic and Applied Microbiology 4:369–381
    [Google Scholar]
  35. Rodriguez-Valera F., Ventosa A., Juez G., Imhoff J. F. 1985; Variation of environmental features and microbial populations with salt concentrations in a multi-pond saltern. Microbial Ecology 11:107–115
    [Google Scholar]
  36. Rüger H. J. 1983; Differentiation of Bacillus globisporus, Bacillus marinus comb, nov., Bacillus aminovorans, and Bacillus insolitus. International Journal of Systematic Bacteriology 33:157–161
    [Google Scholar]
  37. Rüger H. J., Richter G. 1979; Bacillus globisporus subsp. marinus subsp. nov. International Journal of Systematic Bacteriology 29:196–203
    [Google Scholar]
  38. Sieburth J. McN. 1979 Sea Microbes Oxford & New York: Oxford University Press;
    [Google Scholar]
  39. Sneath P. H. A., Johnson R. 1972; The influence on numerical taxonomic similarities of errors in microbiological tests. Journal of General Microbiology 72:377–392
    [Google Scholar]
  40. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy. The Principles and Practice of Numerical Classification San Francisco: Freeman;
    [Google Scholar]
  41. Sokal R. R., Michener C. D. 1958; A statistical method for evaluating systematic relationships. University of Kansas Science Bulletin 38:1409–1438
    [Google Scholar]
  42. Torreblanca M., Rodriguez-Valera F., Juez G., Ventosa A., Kamekura M., Kates M. 1986; Classification of non-alkaliphilic halobacteria based on numerical taxonomy and polar lipid composition, and description of Haloarcula gen. nov. and Halo- ferax gen. nov. Systematic and Applied Microbiology 8:89–99
    [Google Scholar]
  43. Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. 1982; umerical taxonomy of moderately halo- philic Gram-negative rods. Journal of General Microbiology 128:1959–1968
    [Google Scholar]
  44. Ventosa A., Ramos-Cormenzana A., Kocur M. 1982; Moderately halophilic Gram-positive cocci from hypersaline environments. Systematic and Applied Microbiology 4:564–570
    [Google Scholar]
  45. Ventosa A., Rodriguez-Valera F., Poindexter J. S., Reznikoff W. S. 1984; Selection for moderately halophilic bacteria by gradual salinity increases. Canadian Journal of Microbiology 30:1279–1282
    [Google Scholar]
  46. Weeks O. B. 1974; Genus Flavobacterium. In Bergey’s Manual of Determinative Bacteriology, 8th edn. pp. 357–363 Buchanan R. E., Gibbons N. E. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  47. Zeikus J. G., Hegge P. W., Thompson T. E., Phelps T. J., Langworthy T. A. 1983; Isolation and description of Haloanaerobium praevalens gen. nov. and spec, nov., an obligately anaerobic halophile common to Great Salt Lake sediments. Current Microbiology 9:225–234
    [Google Scholar]
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