1887

Abstract

The pool of mRNA stored in dormant sporangiospores of and expressed during early germination in air has been investigated. Total RNA was extracted from dormant and germinating spores and translated in a cell-free rabbit reticulocyte system containing -[S]methionine. Isotopically labelled translation products were analysed by PAGE and autoradiography and were compared with labelled proteins synthesized at the same stages of development. This comparison revealed several significant findings about the fates of individual mRNA populations as templates in translation: (i) a pool of mRNA, presumably represented entirely or in part by a recoverable polyadenylated RNA fraction, can be extracted from dormant spores in a translatable form; (ii) most of the differential gene expression displayed at the level of protein synthesis during germination results from concomitant changes in functional mRNA levels; (iii) some of the stored mRNA species may be activated and others inactivated by post-transcriptional processing mechanisms; and (iv) a small population of gene products may be regulated at the level of selective translation of pre-existing messages.

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/content/journal/micro/10.1099/00221287-133-1-141
1987-01-01
2021-08-03
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References

  1. Alton T. H., Lodish H. F. 1977; Developmental changes in messenger RNAs and protein synthesis in Dictyostelium discoideum. Developmental Biology 60:180–206
    [Google Scholar]
  2. Bartnicki-Garcia S. 1973; Fundamental aspects of hyphal morphogenesis. Symposia of the Society for General Microbiology 23:245–267
    [Google Scholar]
  3. Freer S. N., Van Etten J. L. 1978; Changes in messenger RNAs and protein synthesis during germination of Rhizopus stolonifer sporangiospores. Experimental Mycology 2:313–325
    [Google Scholar]
  4. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  5. Linz J. E., Orlowski M. 1982; Stored mRNA in sporangiospores of the fungus Mucor racemosus. Journal of Bacteriology 150:1138–1144
    [Google Scholar]
  6. Linz J. E., Orlowski M. 1984; Differential gene expression during aerobic germination of Mucor racemosus sporangiospores. Journal of Bacteriology 159:965–972
    [Google Scholar]
  7. O’Farrell P. H. 1975; High resolution two-dimensional electrophoresis of proteins. Journal of Biological Chemistry 250:4007–4021
    [Google Scholar]
  8. Orlowski M. 1979; Changing pattern of cyclic AMP-binding proteins during germination of Mucor racemosus sporangiospores. Biochemical Journal 182:547–554
    [Google Scholar]
  9. Orlowski M., Sypherd P. S. 1978; Regulation of macromolecular synthesis during hyphal germ tube emergence from Mucor racemosus sporangiospores. Journal of Bacteriology 134:76–83
    [Google Scholar]
  10. Papaconstantinou J. 1967; Molecular aspects of lens cell differentiation. Science 156:338–346
    [Google Scholar]
  11. Slater D. W., Spiegelman S. 1966; An estimation of genetic messages in the unfertilized echinoid egg. Proceedings of the National Academy of Sciences of the United States of America 56:164–170
    [Google Scholar]
  12. Stavy L., Gross P. R. 1969; Availability of mRNA from translation during normal and transcription blocked development. Biochimica et biophysica acta 182:203–213
    [Google Scholar]
  13. Sypherd P. S., Borgia P. T., Paznokas J. L. 1979; Biochemistry of dimorphism in the fungus Mucor. Advances in Microbial Physiology 18:68–104
    [Google Scholar]
  14. Tripp M. L., Paznokas J. L. 1981; Relationship between sporulation medium and germination ability of Mucor racemosus sporangiospores. Journal of General Microbiology 127:35–43
    [Google Scholar]
  15. Tripp M. L., Paznokas J. L. 1982a; Glucose-initiated germination of Mucor racemosus sporangiospores. Journal of General Microbiology 128:477–483
    [Google Scholar]
  16. Tripp M. L., Paznokas J. L. 1982b; Role of amino acids and endogenous protein in the germination of Mucor racemosus sporangiospores. Journal of General Microbiology 128:485–495
    [Google Scholar]
  17. Wertman K. F., Paznokas J. L. 1981; Effects of cyclic nucleotides upon the germination of Mucor racemosus sporangiospores. Experimental Mycology 5:314–322
    [Google Scholar]
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