SUMMARY: The mutation in the yeast causes cell cycle arrest late in nuclear division when cells are shifted from the permissive temperature of 25 °C to the restrictive temperature of 36.5 °C. Cell cycle arrest at 36.5 °C is dependent upon the carbon source used: a shift-up in glucose containing media results in cell cycle blockade, whereas a shift-up in ethanol, fructose, glycerol, glycerol plus ethanol, or mannose does not. Metabolite analyses showed accumulation of glucose 6-phosphate in a -bearing strain after a temperature shift-up in glucose-containing medium. Thermal denaturation studies and kinetic measurements indicate the existence of two isoenzymes of phosphoglucose isomerase (EC; one of which is apparently altered in the temperature-sensitive cell cycle mutant. We propose that the gene products of both the and genes are required for cell cycle progression in glucose media and that the gene product has a regulatory function over the gene product.


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