1887

Abstract

A recombinant plasmid was constructed by ligating RI digests of the plasmid cloning vector pBR325 and pZMO2, one of the natural plasmids of ATCC 10988. This vector, named pDS212 (total size 7·9 kb), which was able to transform efficiently, was also transferred to hosts by mobilization during conjugation using the helper plasmid pRK2013. pDS212 was inherited stably in both and hosts and could be recovered intact from them. Markers of pBR325 and pRK2013 were also transferred in but at very low frequencies. Neither pBR325 nor pRK2013 could be recovered intact from the hosts. It is proposed that expression and stability of pDS212 in is due to the origin of replication of pZMO2 that it carries, and that it may be used for developing a gene transfer system in .

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/content/journal/micro/10.1099/00221287-133-1-127
1987-01-01
2022-01-21
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