1887

Abstract

A recombinant plasmid was constructed by ligating RI digests of the plasmid cloning vector pBR325 and pZMO2, one of the natural plasmids of ATCC 10988. This vector, named pDS212 (total size 7·9 kb), which was able to transform efficiently, was also transferred to hosts by mobilization during conjugation using the helper plasmid pRK2013. pDS212 was inherited stably in both and hosts and could be recovered intact from them. Markers of pBR325 and pRK2013 were also transferred in but at very low frequencies. Neither pBR325 nor pRK2013 could be recovered intact from the hosts. It is proposed that expression and stability of pDS212 in is due to the origin of replication of pZMO2 that it carries, and that it may be used for developing a gene transfer system in .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-1-127
1987-01-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/1/mic-133-1-127.html?itemId=/content/journal/micro/10.1099/00221287-133-1-127&mimeType=html&fmt=ahah

References

  1. Bolivar F. 1979; Molecular cloning vehicles derived from the ColEl type plasmid pMB1. Life Sciences 25:807–818
    [Google Scholar]
  2. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heyneker H. L., Boyer H. W., Cross J. H., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2:95–113
    [Google Scholar]
  3. Bringer S., Sahm H., Swyzen W. 1984; Ethanol production by Zymomonas mobilis and its application on an industrial scale. Biotechnology and Bioengineering Symposium 14:311–319
    [Google Scholar]
  4. Browne G. M., Skotnicki M. L., Goodman A. E., Rogers P. L. 1984; Transformation of Zymomonas mobilis by a hybrid plasmid. Plasmid 12:211–214
    [Google Scholar]
  5. Byun M. O. -K., Kaper J. B., Ingram L. O. 1986; Construction of a new vector for the expression of foreign genes in Zymononas mobilis. Journal of Industrial Microbiology 1:9–15
    [Google Scholar]
  6. Carey V. C., Walia S. K., Ingram L. O. 1983; Expression of a lactose transposon (Tn951) in Zymomonas mobilis. Applied and Environmental Microbiology 46:1163–1168
    [Google Scholar]
  7. Cohen S. N., Chang A. C. Y., Hsu L. 1972; Nonchromosomal antibiotic resistance in bacteria: transformation of E. coli by R-factor DNA. Proceedings of the National Academy of Sciences of the United States of America 69:2110–2114
    [Google Scholar]
  8. Dally E. L., Stokes H. W., Eveleigh D. E. 1982; A genetic comparison of strains of Zymomonas mobilis by analysis of plasmid DNA. Biotechnology Letters 4:91–96
    [Google Scholar]
  9. Doelle H. W., Greenfield P. F. 1985a; The production of ethanol from sucrose using Zymomonas mobilis. Applied Microbiology and Biotechnology 22:405–410
    [Google Scholar]
  10. Doelle H. W., Greenfield P. F. 1985b; Fermentation pattern of Zymomonas mobilis at high sucrose concentrations. Applied Microbiology and Biotechnology 22:411–415
    [Google Scholar]
  11. Drainas C., Slater A. A., Coggins L., Montague P., Costa R. G., Ledingham W. M., Kinghorn J. R. 1983; Electron microscopic analysis of Zymomonas mobilis strain ATCC 10988 plasmid DNA. Biotechnology Letters 5:405–408
    [Google Scholar]
  12. Drainas C., Typas M. A., Kinghorn J. R. 1984; A derivative of Zymomonas mobilis ATCC 10988 with impaired ethanol production. Biotechnology Letters 6:37–42
    [Google Scholar]
  13. Friedman A. M., Long S. R., Brown S. E., Buikema W. J., Ausubel F. M. 1982; Construction of a broad host range cosmid vector and its use in the genetic analysis of Rhizobium mutants. Gene 18:289–296
    [Google Scholar]
  14. Kieser T., Hopwood D. A., Wright H. M., Thompson C. T. 1982; pIJ101, a multicopy broad- host-range Streptomyces plasmid: functional analysis and development of DNA cloning vectors. Molecular and General Genetics 185:223–238
    [Google Scholar]
  15. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. 1976; Simple agarose gel electrophoresis method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology 127:1529–1538
    [Google Scholar]
  17. Rogers P. L., Goodman A. E., Heyes R. H. 1984; Zymomonas ethanol fermentations. Microbiological Sciences 1:133–136
    [Google Scholar]
  18. Skotnicki M. L., Tribe D. E., Rogers P. L. 1980; R-plasmid transfer in Zymomonas mobilis. Applied and Environmental Microbiology 40:7–12
    [Google Scholar]
  19. Swings J., De Ley J. 1977; The biology of Zymomonas. Bacteriological Reviews 41:1–46
    [Google Scholar]
  20. Tonomura K., Kurose N., Konishi S., Kawasaki H. 1982; Occurrence of plasmids in Zymomonas mobilis. Agricultural and Biological Chemistry 46:2851–2853
    [Google Scholar]
  21. Tonomura K., Okamoto T., Yasui M., Yanase H. 1986; Shuttle vectors for Zymomonas mobilis. Agricultural and Biological Chemistry 50:805–808
    [Google Scholar]
  22. Viikari L. 1984a; Formation of levan and sorbitol from sucrose by Zymomonas mobilis. Applied Microbiology and Biotechnology 19:252–255
    [Google Scholar]
  23. Viikari L. 1984b; Formation of Sorbitol by Zymomonas mobilis. Applied Microbiology and Biotechnology 20:118–123
    [Google Scholar]
  24. Viikari L., Linko M. 1986; Rate and yield limiting factors in continuous fermentation of sucrose by Zymomonas mobilis. Biotechnology Letters 8:139–144
    [Google Scholar]
  25. Walia S. K., Carey V. C., All B. P. III Ingram L. O. 1984; Self-transmissible plasmid in Zymomonas mobilis carrying antibiotic resistance. Applied and Environmental Microbiology 47:198–200
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-1-127
Loading
/content/journal/micro/10.1099/00221287-133-1-127
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error