1887

Abstract

The unicellular cyanobacterium contains both photoreactivation and excision repair mechanisms for correcting UV-induced damage to its cellular DNA. An 11·5 kb RI fragment was isolated from a cosmid bank of and was shown to complement a deletion in S. 17 and JC10289. These strains showed increased survival to UV and methyl methanesulphonate (MMS) when transformed with the cyanobacterial DNA fragment, and also showed filamentation in response to UV irradiation. Preliminary analysis of the protein encoded by the cyanobacterial DNA fragment indicated a major protein of 39000 Da; this is very similar in size to the protein of .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-133-1-119
1987-01-01
2022-01-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/133/1/mic-133-1-119.html?itemId=/content/journal/micro/10.1099/00221287-133-1-119&mimeType=html&fmt=ahah

References

  1. Allen M. M. 1968; Simple conditions for the growth of unicellular blue-green algae on plates. Journal of Phycology 4:1–4
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Hohn B. 1979; In vitro packaging of λ and cosmid DNA. Methods in Enzymology 68:299–309
    [Google Scholar]
  4. Jensen T. E., Sicko L. M. 1973; Fine structure of the cell wall of Gloeocapsa alpicola, a blue-green alga. Cytobiology 6:439–446
    [Google Scholar]
  5. Kumar H. D. 1963; Effects of radiations on blue- green algae: the production and characterization of a strain of Anacystis nidulans resistant to ultraviolet radiation. Annals of Botany 27:723–733
    [Google Scholar]
  6. Little J. W., Mount D. W. 1982; The SOS regulatory system of Escherichia coli. Cell 29:11–22
    [Google Scholar]
  7. Little J. W., Mount D. W., Yanis-Perron C. R. 1981; Purified LexA protein is a repressor of the lex A and recA genes. Proceedings of the National Academy of Sciences of the United States of America 78:4199–4203
    [Google Scholar]
  8. Maniatas T., Fritsch E. G., Sambrook J. 1982 Molecular Cloning, a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. Marsden H. S., Pollard E. C., Ginoza W., Randall E. P. 1974; Involvement of recA and exr genes in the in vitro inhibition of the recBC nuclease. Journal of Bacteriology 118:465–470
    [Google Scholar]
  10. O’Brien P. A., Houghton J. A. 1982a; Photoreactivation and excision repair of UV induced pyrimidine dimers in the unicellular cyanobacterium Gloeocapsa alpicola (Synechocystis PCC 6308). Photochemistry and Photobiology 35:359–364
    [Google Scholar]
  11. O’Brien P. A., Houghton J. A. 1982b; UV induced DNA degradation in the cyanobacterium Synechocystis PCC 6308. Photochemistry and Photobiology 36:417–422
    [Google Scholar]
  12. Rippka R., Deruelles J., Waterbury J. B., Herdman M., Stanier R. Y. 1979; Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111:1–61
    [Google Scholar]
  13. Saito N., Werbin H. 1970; Purification of a blue- green algal deoxyribonucleic acid photoreactivating enzyme.An enzyme requiring light as a physical cofactor to perform its catalytic function. Biochemistry 3:2610–2620
    [Google Scholar]
  14. Sancar A., Stachelek C., Konigsberg W., Rupp W. D. 1980; Sequences of the recA gene and protein. Proceedings of the National Academy of Sciences of the United States of America 77:2611–2615
    [Google Scholar]
  15. Satta G., Gudas L., Pardee A. B. 1979; Degradation of E. coli DNA: evidence for limitation in vivo by protein X, the recA gene product. Molecular and General Genetics 168:69–80
    [Google Scholar]
  16. Simon R., Priefer U., Puhler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Biotechnology 1:784–791
    [Google Scholar]
  17. Tang T., Asato Y. 1978; Ultraviolet light induction and photoreactivation of thymine dimers in the cyanobacterium Anacystis nidulans. Archives of Microbiology 118:193–197
    [Google Scholar]
  18. Werbin H., Rupert C. S. 1968; Presence of photoreactivating enzyme in blue-green algal cells. Photochemistry and Photobiology 7:225–230
    [Google Scholar]
  19. Williams E., Lambert J., O’Brien P., Houghton J. A. 1979; Evidence for dark repair of ultraviolet light damage in the blue-green alga Gloeocapsa alpicola. Photochemistry and Photobiology 29:543–547
    [Google Scholar]
  20. Witkin E. 1976; Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli. Bacteriological Reviews 40:869–907
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-133-1-119
Loading
/content/journal/micro/10.1099/00221287-133-1-119
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error