1887

Abstract

Summary: Plasma membranes from osmotically lysed protoplasts of were concentrated 21 to 26-fold (using the recovery of [H]dansyl chloride and ATPase activity at pH 6.5, as criteria). The contamination by mitochondria was approximately 10%. The plasma membrane ATPase was stimulated by K by up to 350% at pH 6.5; Na had a lesser effect, whereas Li had none. The stimulation by K was independent of Cl, NO or CO as accompanying anions. The pH optimum of the ATPase was narrowed in the presence of 80 m-KC1 to a distinct peak at pH 6.5. Under these conditions nucleoside triphosphates other than ATP were hydrolysed at rates less than 5% of that with ATP, and the of the ATPase for ATP was lowered from 1.3 to 0.6 m. Orthovanadate (40 μ) and oligomycin (5 μg ml) inhibited the plasma membrane ATPase by 65% and by 20%, respectively. In contrast, the ATPase activity of the mitochondrial fraction had a sharp optimum at pH 8.5 and was not stimulated by added K.

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1986-09-01
2021-05-17
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References

  1. Ahlbrs J. 1984; Effect of different salts on the plasma membrane ATPase and on proton transport in yeast. Canadian Journal of Biochemistry and Cell Biology 62:998–1005
    [Google Scholar]
  2. Aldbrmann B., Höfbr M. 1981; The active transport of monosaccharides by the yeast Metschnikowia reukaufii: evidence for an electrochemical gradient of H+ across the cell membrane. Experimental Mycology 5:120–132
    [Google Scholar]
  3. Aldbrmann B., Höfer M. 1984; Fractionation of membranes from Metschnikowia reukaufii proto-plasts. Evidence for a plasmamembrane-bound ATPase. journal of general Microbiology 130:711–723
    [Google Scholar]
  4. Blasco F., Chapuis J. P., Giordani R. 1981; Characterisation of plasma membrane ATPase of Candida tropicalis. Biochimie 63:507–514
    [Google Scholar]
  5. Bowman B. J., Slayman C. W. 1977; Characterisation of plasma membrane ATPase of Neurospora crassa. Journal of Biological Chemistry 252:3357–3363
    [Google Scholar]
  6. Delhez J., Dufour J. P., Thines P., Goffeau A. 1977; Comparison of the properties of plasma membrane bound and mitochondria bound ATPase in the yeast Schizosaccharomyces pombe. European Journal of Biochemistry 79:319–328
    [Google Scholar]
  7. Eddy A. A. 1982; Mechanism of solute transport in selected eukaryotic micro-organisms. Advances in Microbial Physiology 23:2–78
    [Google Scholar]
  8. Giaquinta R. 1979; Phloem loading of sucrose. Plant Physiology 63:744–748
    [Google Scholar]
  9. Goffeau A., Slayman c. w. 1981; The proton translocating ATPase of the fungal plasma membrane. Biochimica et biophysica acta 639:197–223
    [Google Scholar]
  10. Hauer R., Höfbr M. 1978; Evidence for interactions between the energy-dependent transport of sugars and the membrane-potential in the yeast Rhodotorula gracilis. Journal of Membrane Biology 43:335–349
    [Google Scholar]
  11. Hauer R., Uhlbmann G., Neumann J., Höfer M. 1981; Proton pumps oFf the plasma membrane of the yeast Rhodotorula gracilis. Their coupling to fluxes of potassium and other ions. Biochemical et biosphysica acta 649:680–690
    [Google Scholar]
  12. Höfer M., Misra P. C. 1978; Evidence for a proton/sugar symport in the yeast Rhodotorula gracilis (glutinis). Biochemical Journal 172:15–22
    [Google Scholar]
  13. Komor E. 1973; Proton-coupled hexose transport in Chlorella vulgaris. FEBS Letters 38:16–18
    [Google Scholar]
  14. Künemund A., Höfer M. 1983; Passive fluxes of K+ and H+ in wild strain and nystatin-resistant mutant of Rhodotorula gracilis (ATCC 261944). Biochimica et biophysica acta 735:203–210
    [Google Scholar]
  15. Marrè E., Ballarin-Denti A. 1985; The proton pumps of the plasmalemma and the tonoplast in higher plants. Journal of Bioenergetics and Biomembranes 17:1–21
    [Google Scholar]
  16. Mitchell P. 1966; Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biological Reviews 41:445–502
    [Google Scholar]
  17. Novacky A., Ullrich-Eberius C. I., Lüttge U. 1978; Membrane potential changes during transport of hexose in Lemna gibba G 1. Planta 138:263–270
    [Google Scholar]
  18. Poole R. J. 1978; Energy coupling for membrane transport. Annual Review of Plant Physiology 30:437–460
    [Google Scholar]
  19. Scarborough G. A. 1976; The Neurospora plasma membrane ATPase is an electrogenic pump. Proceedings of the National Academy of Sciences of the United States of America 73:1485–1488
    [Google Scholar]
  20. Schmidt R., Ackermann R., Kratky Z., Wassbrman B., Jacobwn B. 1983; Fast and efficient purification of yeast plasma membranes using cationic silica microbeads. Biochimica et biophysica acta 732:421–427
    [Google Scholar]
  21. Serrano R. 1978; Characterisation of the plasma membrane ATPase of Saccharomyces cerevisiae. Molecular and Cellular Biochemistry 22:51–63
    [Google Scholar]
  22. Spanswick R. M. 1981; Electrogenic ion pumps. Annual Review of Plant Physiology 32:267–289
    [Google Scholar]
  23. Tanner W., Komor E., Fenzl F., Decker M. 1977; Sugar-proton cotransport systems. Regulation of Cell Membrane Activities in Plants pp. 79–80 Edited by Marrè E., Ciferri O. Amsterdam: Elsevier;
    [Google Scholar]
  24. Villalobo A. 1982; Potassium transport coupled to ATP hydrolysis in reconstituted proteoliposomes of yeast plasma membrane ATPase. Journal of Biological Chemistry 257:1824–1828
    [Google Scholar]
  25. Villalobo A., Boutry M., Goffeau A. 1981; Electrogenic proton translocation coupled to ATP hydrolysis by the plasma membrane Mg2+-dependent ATPase of yeast in reconstituted proteolipo-somes. Journal of Biological Chemistry 256:12081–12087
    [Google Scholar]
  26. West I. c., Mitchell P. 1972; Proton coupled β galactoside translocation in non-metabolizing Escherichia coli. Journal of Bioenergetics and Biomembranes 3:445–462
    [Google Scholar]
  27. Willsky G. R. 1979; Characterisation of the plasma membrane Mg2+-ATPase from the yeast Saccharomyces cerevisiae. Journal of Biological Chemistry 254:3326–3332
    [Google Scholar]
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