Isolation and Chemical Characterization of the Sheath from the Cyanobacterium SAG B.41.79 Free

Abstract

Summary: The sheath of the unicellular cyanobacterium SAG B.41.79 was isolated from a crude cell envelope fraction by discontinuous sucrose gradient centrifugation, and was further purified by treatment with lysozyme followed by Triton X-100 or sodium dodecyl sulphate (SDS) extraction. The absence of muramic and diaminopimelic acids and of β-hydroxy fatty acid showed the fraction to be free from cell wall components. The sheath had a fibrillar fine structure with the fibres parallel to the cell surface. The total neutral sugar content was 45.9% (w/w). The main sugars were glucose and 2--methyl-6-deoxyhexose. Additional -methyl sugars, 2--methylhexose, 3--methylhexose and a 2--methyl sugar (not further identified), were present. Protein could not be completely removed from the sheath fraction by treatment with boiling SDS. The contents of fatty acids, phosphorus, uronic acids and glucosamine in the fraction were all less than 0.5% (w/w).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-9-2595
1986-09-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/9/mic-132-9-2595.html?itemId=/content/journal/micro/10.1099/00221287-132-9-2595&mimeType=html&fmt=ahah

References

  1. Cox G., Benson D., Dwarte D. M. 1981; Ultrastructure of a cave-wall cyanophyte – Gloeocapsa NS4. Archives of Microbiology 130:165–174
    [Google Scholar]
  2. Drews G., Weckesser J. 1982; Function, structure and composition of cell wall and external layers. The Biology of Cyanobacteria pp. 333–357 Edited by Carr N. G., Whitton B. A. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  3. Dunn J. H., Wolk C. P. 1970; Composition of cellular envelopes of Anabaena cylindrica. Journal of Bacteriology 103:153–158
    [Google Scholar]
  4. Fuhs G. W. 1958; Enzymatischer Abbau der Membranen von Oscillatoria amoena (Kütz) Gomont mit Lysozym. Archiv für Mikrobiologie 29:51–52
    [Google Scholar]
  5. Golecki J. R. 1977; Studies on ultrastructure and composition of cell walls of the cyanobacterium Anacystis nidulans. Archives of Microbiology 114:35–41
    [Google Scholar]
  6. Golecki J. R., Drews G. 1974; Zur Struktur der Blaualgen-Zellwand. Gefrierätzuntersuchungen an normalen und extrahierten Zellwänden von Anabaena variabilis. Cytobiologie 8, 213–227
    [Google Scholar]
  7. Jansson P. E., Kenne L., Liedgren H., Lindberg B., Lönngren J. 1976; A practical guide to the methylation analysis of carbohydrates. Chemical Communications 1976, no 8:1–74
    [Google Scholar]
  8. Jensen T. E., Sicko L. M. 1972; The fine structure of the cell wall of Gloeocapsa alpicola, a blue-green alga. Cytobiologie 4:439–446
    [Google Scholar]
  9. Kellenberger E., Ryter A., Sechaud J. 1958; Electron microscope study of DNA containing plasm. Journal of Biophysical and Biochemical Cytology 4:671–678
    [Google Scholar]
  10. Kickhöfen B., Warth R. 1968; Eine Trennkammer für die Hochspannungselektrophorese nach dem Michel’schen Prinzip. Journal of Chromatography 33:558–560
    [Google Scholar]
  11. Lowry O. H., Roberts N. R., Leiner K. Y., Wu M. L., Farr A. L. 1954; The quantitative histochemistry of brain I. Chemical methods. Journal of Biological Chemistry 207:1–17
    [Google Scholar]
  12. Potts M., Ocampo-Friedmann R., Bowman M. A., Tözün B. 1983; Chroococcus S24 and Chroococcus N41 (cyanobacteria): morphological, biochemical and genetical characterization and effects of water stress on ultrastructure. Archives of Microbiology 135:81–90
    [Google Scholar]
  13. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  14. Rippka R., Deruelles J., Waterbury J. B., Herdman M., Stanier R. Y. 1979; Generic assignments, strain histories and properties of pure cultures of cyanobacteria. Journal of General Microbiology 111:1–61
    [Google Scholar]
  15. Schmidt W., Drews G., Weckesser J., Fromme I., Borowiak D. 1980; Characterization of lipopolysaccharides from eight strains of the cyanobacterium Synechococcus. Archives of Microbiology 127:209–215
    [Google Scholar]
  16. Schnaitman C. A. 1971; Effect of ethylenediamine-tetraacetic acid, Triton X-100 and lysozyme on the morphology and chemical composition of isolated cell wall of Escherichia coli. Journal of Bacteriology 108:553–562
    [Google Scholar]
  17. Schrader M., Drews G., Golecki J. R., Weckesser J. 1982 a; Isolation and characterization of sheath from the cyanobacterium Chlorogloeopsis PCC 6912. Journal of General Microbiology 128:267–272
    [Google Scholar]
  18. Schrader M., Drews G., Weckesser J., Mayer H. 1982 b; Polysaccharide containing 6-O-methyl-D-mannose in Chlorogloeopsis PCC 6912. Journal of General Microbiology 128:273–277
    [Google Scholar]
  19. Stanier R. Y., Cohen-Bazire G. 1977; Photo-trophic procaryotes: the cyanobacteria. Annual Review of Microbiology 31:225–274
    [Google Scholar]
  20. Trevelyan W. E., Procter D. P., Harrison J. S. 1950; Detection of sugars on paper chromatograms. Nature, London 166:444–445
    [Google Scholar]
  21. Vaara T. 1982; The outermost surface structures in the chroococcacean cyanobacteria. Canadian Journal of Microbiology 28:924–941
    [Google Scholar]
  22. Watson M. L. 1958; Staining of tissue sections for electron microscopy with heavy metals. Journal of Biophysics, Biochemistry and Cytology 4:475–478
    [Google Scholar]
  23. Weckesser J., Drews G., Mayer H. 1979; Lipopolysaccharides of photosynthetic prokaryotes. Annual Review of Microbiology 33:215–239
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-9-2595
Loading
/content/journal/micro/10.1099/00221287-132-9-2595
Loading

Data & Media loading...

Most cited Most Cited RSS feed