Summary: Among the spheroidal fission-yeast (Schizosaccharomyces pombe) cells resulting from aculeacin A treatment were found cells whose putative growth axis and polarity differed from those of their progenitor, that is they were changed in the orientation of their septum. The ratio of cell length (measured perpendicular to the septum plane) to septum diameter of these cells equalled or exceeded unity without exception, whether the septum orientation changed or not and whether the cellular shape was spherical, spheroidal or cylindrical. From these results we conclude that the septum is always oriented perpendicular to the plane including the longest axis of the cell even if the morphology is irregular or the new septum has become perpendicular to the previous septum. There is no cellular region forbidden to new septa.
JohnsonB. F.,
McdonaldI. J.1983; Cell division: a separable cellular sub–cycle in the fission yeast Schizosaccharomyces pomhe. Journal of General Microbiology 129:3411–3419
MccullyE. K.,
RobinowC. F.1971; Mitosis in the fission yeast Schizosaccharomyces pombe: a comparative study with light and electron microscopy. Journal of Cell Science 9:475–507
MinetM.,
NurseP.,
ThuriauxP.,
MitchisonJ. M.1979; Uncontrolled septation in a cell division cycle mutant of the fission yeast Schizosaccharomyces pomhe. Journal of Bacteriology 137:440–446
MiyataM.,
KitamuraJ.,
MiyataH.1980; Lysis of growing fission yeast cells induced by aculeacin A, a new antifungal antibiotic. Archives of Microbiology 127:l–16
MiyataM.,
KanbeT.,
TanakaK.1985; Morphological alterations of the fission yeast Schizosaccharomyces pombe in the presence of aculeacin A: spherical wall formation. Journal of General Microbiology 131:611–621
MiyataM.,
MiyataH.,
JohnsonB. F.1986; Asymmetric location of the septum in morphologically altered cells of the fission yeast Schizosaccharomyces pombe. Journal of General Microbiology 132:883–891
MizoguchiJ.,
SaitoT.,
MizunoK.,
HayanoK.1977; On the mode of action of a new antifungal antibiotic, aculeacin A: inhibition of cell wall synthesis in Saccharomyces cerevisiae. Journal of Antibiotics 30:308–313
NasmythK.,
NurseP.1981; Cell division cycle mutant altered in DN A replication and mitosis in the fission yeast, Schizosaccharomyces pombe. Molecular and General Genetics 182:119–124
NurseP.,
FantesP. A.1981; Cell cycle controls in fission yeast: a genetic analysis. Cell Cycle pp. 85–98 Edited by
JohnP. C. L.
Cambridge: Cambridge University Press;
NurseP.,
ThuriauxP.,
NasmythK.1976; Genetic control of the cell division cycle in the fission yeast Schizosaccharomyces pombe. Molecular and General Genetics 146:167–178
PetersonJ. B.,
RisH.1976; Electron–microscopic study of the spindle and chromosome movement in the yeast Saccharomyces cerevisiae. Journal of Cell Science 22:219–242
TanakaK.,
KanbeT.1986; Mitosis in the fission yeast Schizosaccharomyces pombe as revealed by freeze substitution electron microscopy. Journal of Cell Science 80:253–268