RT Journal Article SR Electronic(1) A1 McGillivray, Ann M. A1 Gow, Neil A. R.YR 1986 T1 Applied Electrical Fields Polarize the Growth of Mycelial Fungi JF Microbiology, VO 132 IS 9 SP 2515 OP 2525 DO https://doi.org/10.1099/00221287-132-9-2515 PB Microbiology Society, SN 1465-2080, AB Summary: Mycelial fungi generate endogenous electrical fields which are associated with polarized tip growth. Here we show that applied electrical fields can dramatically affect the polarity of growth of a variety of filamentous fungi including Neurospora crassa, Aspergillus nidulans, Mucor mucedo, Trichoderma harzianum and Achlya bisexualis. The precise behaviour of each fungus when exposed to an electric field was different; however the sites of germ tube formation and branching, the direction of hyphal extension and the frequency of branching and germination could all be affected. N. crassa and Ach. bisexualis grew and formed branches towards the anode while Asp. nidulans and M. mucedo exhibited tropisms towards the cathode. Galvanotropism of hyphae and branches of T. harzianum was in opposite directions. Germ tube formation from conidia of N. crassa was highly polarized in electrical fields whereas fields of a similar strength had little effect on the polarity of germ tube formation in Asp. nidulans. Hyphae became aligned perpendicularly as they grew longer and as the field strength increased. It is suggested that this perpendicularly orientated extension relieves growth-inhibiting perturbations of the membrane potential in cells which had been aligned parallel to the field. These results are discussed in relation to the hypothesis that the polarity of hyphal growth is under electrical control., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-9-2515