Utilization of Amino Acids and Lack of Diazotrophy in the Thermophilic Anoxygenic Phototroph Free

Abstract

Summary: Four strains of the thermophilic phototrophic green bacterium were tested for nitrogenase activity under a variety of nutritional conditions consistent with nitrogenase derepression in anoxygenic photosynthetic bacteria. Although all strains of could grow with various amino acids as sole nitrogen sources, no growth on N was observed; acetylene reduction was undetectable in cells grown under any nutritional conditions. The inability of to fix N is discussed in connection with its thermophilic character.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-9-2469
1986-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/9/mic-132-9-2469.html?itemId=/content/journal/micro/10.1099/00221287-132-9-2469&mimeType=html&fmt=ahah

References

  1. Bauld J., Brock T. D. 1973; Ecological studies of Chloroflexis, a gliding photosynthetic bacterium. Archiv fur Mikrobiologie 92:267–284
    [Google Scholar]
  2. Bottomley P. J., Grillo J. F., van Baalen C., Tabita F. R. 1979; Synthesis of nitrogenase and heterocysts by Anabaena sp CA in the presence of high levels of ammonia. Journal of Bacteriology 140:938–943
    [Google Scholar]
  3. Brock T. D. 1978; Thermophilic Microorganisms and Life at High Temperatures. New York: Spring:Verlag
    [Google Scholar]
  4. Bruce B. D., Fuller R. C., Blankenship R. E. 1982; Primary photochemistry in the facultatively aerobic green photosynthetic bacterium Chloroflexus aurantiacus. Proceedings of the National Academy of Sciences of the United States of America 79:6532–6536
    [Google Scholar]
  5. Castenholz R. W. 1969; Thermophilic blue-green algae and the thermal environment. Bacteriological Reviews 33:476–504
    [Google Scholar]
  6. Drutschmann M., Klemme J.-H. 1985; Sulfide repressed membrane bound hydrogenase in the thermophilic facultative phototroph Chloroflexus aurantiacus.> . FEMS Microbiology Letters 28:231–235
    [Google Scholar]
  7. Gest H., Kamen M. D. 1949; Photoproduction of molecular hydrogen by Rhodospirillum rubrum. Science 109:558–559
    [Google Scholar]
  8. Gest H., Kamen M. D. 1960; The photosynthetic bacteria. In Encyclopedia of Plant Physiology V/2: pp 568–612
    [Google Scholar]
  9. Heda G. D., Madigan M. T. 1986; Aspects of nitrogen fixation in Chlorobium. Archives of Microbiology 143:330–336
    [Google Scholar]
  10. Hennecke H., Shanmugam K. T. 1979; Temperature control of nitrogen fixation in Klebsiella pneumoniae. Archives of Microbiology 123:259–265
    [Google Scholar]
  11. Jackson J. E., Castenholz R. W. 1975; Fidelity of thermophilic blue-green algae to hot spring habitats. Limnology and Oceanography 20:305–322
    [Google Scholar]
  12. Kamen M. D., Gest H. 1949; Evidence for a nitrogenase system in the photosynthetic bacterium Rhodospirillum rubrum. Science109–560
    [Google Scholar]
  13. Madigan M. T. 1984; A novel photosynthetic purple bacterium isolated from a Yellowstone hot spring. Science 225:313–315
    [Google Scholar]
  14. Madigan M. T. 1986; Chromatium tepidum, spn., a thermophilic photosynthetic bacterium of the family Chromatiaceae. International Journal of Systematic Bacteriology 36:222–227
    [Google Scholar]
  15. Madigan M. T., Cox S. S., Stegeman R. A. 1984; Nitrogen fixation and nitrogenase activities in members of the family Rhodospirillaceae. Journal of Bacteriology 157:73–78
    [Google Scholar]
  16. Madigan M. T., Petersen S. R., Brock T. D. 1974; Nutritional studies on Chloroflexus, a filamentous, photosynthetic gliding bacterium. Archives of Microbiology 100:97–103
    [Google Scholar]
  17. Pierson B. K., Castenholz R. W. 1974a; A phototrophic gliding filamentous bacterium of hot springs, Chloroflexus aurantiacus, gen and sp. nov. Archives of Microbiology 100:5–24
    [Google Scholar]
  18. Pierson B. K., Castenholz R. W. 1974b; Studies of pigments and growth in Chloroflexus aurantiacus, a phototrophic filamentous bacterium. Archives of Microbiology 100:283–305
    [Google Scholar]
  19. Pierson B. K., Thornber J. P. 1983; Isolation and spectral characterization of photochemical reaction centers from the thermophilic green bacterium, Chloroflexus aurantiacus J-10-f1. Proceedings of the National Academy of Sciences of the United States of America 80:80–84
    [Google Scholar]
  20. Postgate J. R. 1971 editor Fixation by fr living microbes: physiology
    [Google Scholar]
  21. Postgate J. R., Kent H. M. 1985; Diazotrophy within Desulfovibrio. Journal of General Microbiology 131:2119–2122
    [Google Scholar]
  22. Stewart W. D. P. 1970; Nitrogen fixation by blue green algae in Yellowstone thermal areas. Phycologia 9:261–268
    [Google Scholar]
  23. Stevens S. E., Mehta V. B., Lane L. S. 1985; Heterocyst development and nitrogen fixation by Mastigocladus laminosus. In Nitrogen Fixation and CO2 Metabolism pp 235–243
    [Google Scholar]
  24. Yoch D. C. 1978; Nitrogen fixation and hydrogen metabolism by photosynthetic bacteria. In The Photosynthetic Bacteria pp 657–676
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-9-2469
Loading
/content/journal/micro/10.1099/00221287-132-9-2469
Loading

Data & Media loading...

Most cited Most Cited RSS feed