1887

Abstract

Summary: The effect of the nitrogen source on the activity of ferredoxin-dependent nitrite reductase has been studied in the cyanobacterium synthesis of nitrite reductase occurred in the absence of an added nitrogen source, although enzyme activity was higher when the medium contained either NO or NO . The positive effect of NO on nitrite reductase was also evident in tungstate-treated , which lacked an active nitrate reductase, indicating that the stimulatory effect was due to NO itself and not to the NO resulting from its intracellular reduction. NH acted as a repressor, overriding any positive effect of NO or NO . Nitrite reductase synthesis was freed from NH repression by -methionine--sulphoximine, an irreversible inhibitor of glutamine synthetase. NH must therefore be metabolized through glutamine synthetase before repressing nitrite reductase. The kinetics of nitrate reductase and nitrite reductase development were similar in cells transferred from NH - to NO -containing media, suggesting a coordinate regulation of synthesis.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-9-2463
1986-09-01
2024-12-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/9/mic-132-9-2463.html?itemId=/content/journal/micro/10.1099/00221287-132-9-2463&mimeType=html&fmt=ahah

References

  1. Candau P. 1979; Purificación n y propiedades de la ferredoxina-nitrato reductasa de la cianobacteria Anacystis nidulans. PhD thesis, University of Sevilla, Sevilla, Spain. (In Spanish)
    [Google Scholar]
  2. Candau P., Manzano C., Losada M. 1976; Bioconversion of light energy into chemical energy through reduction with water of nitrate to ammonia. Nature, London 262:715–717
    [Google Scholar]
  3. Flores E., Guerrero M. G., Losada M. 1980; Short-term ammonium inhibition of nitrate utilization by Anacystis nidulans and other cyanobacteria. Archives of Microbiology 128:137–144
    [Google Scholar]
  4. Flores E., Guerrero M. G., Losada M. 1983a; Photosynthetic nature of nitrate uptake and reduction in the cyanobacterium Anacystis nidulans. Biochimica et biophysica acta 722:408–416
    [Google Scholar]
  5. Flores E., Ramos J. L., Herrero A., Guerrero M. G. 1983b; Nitrate assimilation by cyanobacteria. In Photosynthetic Prokaryotes: Cell Differentiation and Function pp 363–387
    [Google Scholar]
  6. Guerrero M. G., Vega J. M., Losada M. 1981; The assimilatory nitrate-reducing system and its regulation. Annual Review of Plant Physiology 32:169–204
    [Google Scholar]
  7. Hattori A., Myers J. 1966; Reduction of nitrate by subcellular preparations of Anabaena cylindrica I. Reduction of nitrite to ammonia. Plant Physiology 41:1031–1036
    [Google Scholar]
  8. Hattori A., Myers J. 1967; Reduction of nitrate and nitrite by subcellular preparations of Anabaena cylindrica II. Reduction of nitrate to nitrite. Plant and Cell Physiology 8:327–337
    [Google Scholar]
  9. Hattori A., Uesugi I. 1968; Purification and properties of nitrite reductase from the blue-green alga Anabaena cylindrica. Plant and Cell Physiology 9:689–699
    [Google Scholar]
  10. Herrero A., Flores E., Guerrero M. G. 1981; Regulation of nitrate reductase levels in the cyanobacteria Anacystis nidulans, Anabaena sp. strain 7119, and Nostoc sp. strain 6719. Journal of Bacteriology 145:175–180
    [Google Scholar]
  11. Herrero A., Flores E., Guerrero M. G. 1984; Regulation of the nitrate reductase level in Anacystis nidulans: activity decay under nitrogen stress. Archives of Biochemistry and Biophysics 234:454–459
    [Google Scholar]
  12. Herrero A., Flores E., Guerrero M. G. 1985; Regulation of nitrate reductase cellular levels in the cyanobacteria Anabaena variabilis and Synechocystis sp. FEMS Microbiology Letters 26:21–25
    [Google Scholar]
  13. Manzano C., Candau P., Gómez-Moreno C., Relimpio A. M., Losada M. 1976; Ferredoxindependent photosynthetic reduction of nitrate and nitrite by particles of Anacystis nidulans. Molecular and Cellular Biochemistry 10:161–169
    [Google Scholar]
  14. Méndez J. M., Vega J. M. 1981; Purification and molecular properties of nitrite reductase from Anabaena sp 7119. Physiologia plantarum 52:7–14
    [Google Scholar]
  15. Méndez J. M., Hérrero A., Vega J. M. 1981; Characterization and catalytic properties of nitrite reductase from Anabaena sp 7119. Zeitschrift fur Pflanzenphysiologie 103:305–315
    [Google Scholar]
  16. Mikami B., Ida S. 1984; Purification and properties of ferredoxin-nitrate reductase from the cyanobacterium Plectonema boryanum. Biochimica et biophysica acta 791:294–304
    [Google Scholar]
  17. Ohmori K., Hattori A. 1970; Induction of nitrate and nitrite reductases in Anabaena cylindrica. Plant and Cell Physiology 11:873–878
    [Google Scholar]
  18. Ortega T., Castillo F., Cárdenas J. 1976; Photolysis of water coupled to nitrate reduction by Nostoc muscorum subcellular particles. Biochemistry and Biophysics Research Communications 71:885–891
    [Google Scholar]
  19. Peschek G. A. 1979; Nitrate and nitrite reductase and hydrogenase in Anacystis nidulans grown in Fe-and Mo-deficient media. FEMS Microbiology Letters 6:371–374
    [Google Scholar]
  20. Snell F. D., Snell C. T. 1949 Colorimetric Methods of Analysis 3: pp 804–805
    [Google Scholar]
/content/journal/micro/10.1099/00221287-132-9-2463
Loading
/content/journal/micro/10.1099/00221287-132-9-2463
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error