Summary: 1,3-β-D-Glucanase and chitinase activities were induced in Trichoderma harzianum when glucose or cell walls of Rhizoctonia solani (anastomosis group AG2) were used as sole carbon source. Electrophoresis showed that more proteins were induced by cell walls than by glucose. The composition, as revealed by electrophoresis, of the T. harzianum extracellular proteins was similar when grown on R. solani AG2 and AG4 cell walls but different for R. solani AG1 cell walls. Several major proteins were induced in all strains of T. harzianum but there were strain differences in the number and intensity of other proteins. The number of induced proteins was less for four strains of T. viride and their composition was different from those of five strains of T. harzianum. The results indicate that chitinase, 1,3-β-D-glucanase and a large number of other extracellular proteins may be involved in the degradation of R. solani cell walls.
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