1887

Abstract

Peptidase D of , cleaving the unusual dipeptide carnosine, was found to be encoded by the ColE1 hybrid plasmid pLC44-11. From this plasmid the gene was subcloned into small vectors. As shown by successive reduction of the flanking sequences of genomic DNA, the order of genes in the region at 6 min of the K12 map is , in the clockwise orientation. Insertional inactivation of the gene and expression of recombinant plasmids in maxicells allowed the identification of the product as a 52 kDa protein. Comparison with the 100 kDa protein molecular mass determined by gel filtration suggests that active peptidase D is probably a dimer.

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1986-08-01
2022-01-23
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