1887

Abstract

Summary: The cellulase gene from the alkalophilic sp. strain 1139 was cloned in using pBR322. Plasmid pFK1 was isolated from transformants producing cellulase, and the cloned cellulase gene was found to be in a 4.6 kb dIII fragment. The cellulase gene was subcloned in a functional state on a 2.9 kb DNA fragment and its nucleotide sequence was determined. The coding sequence showed an open reading frame encoding 800 amino acids. The pFK1-encoded cellulase had the same enzymic properties as the extracellular cellulase produced by the alkalophilic sp. strain 1139, but its was slightly higher.

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/content/journal/micro/10.1099/00221287-132-8-2329
1986-08-01
2022-01-24
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References

  1. Béguin P., Cornet P., Aubert J.-P. 1985; Sequence of a cellulase gene of the thermophilic bacterium Clostridium thermocellum. Journal of Bacteriology 162:102–105
    [Google Scholar]
  2. Bolivar F., Rodriguez R. L., Greene P. J., Betlach M. C., Heynecker H. L., Boyer H. W. 1977; Construction and characterization of new cloning vehicles. II.. A multipurpose cloning system Gene 2:95–113
    [Google Scholar]
  3. Cornet P., Tronik D., Millet J., Aubert J.-P. 1983; Cloning and expression in Escherichia coli of Clostridium thermocellum genes coding for amino acid synthesis and cellulose hydrolysis. FEMS Microbiology Letters 16:137–141
    [Google Scholar]
  4. Davis R. W., Botstein D., Roth J. R. 1980; A Manual for Genetic Engineering. Advanced Bacterial Genetics pp. 120–121 Cold Spring Harbor, New York :: Cold Spring Harbor Laboratory;
    [Google Scholar]
  5. Edman P., Henschen A. 1975; Sequence determination. In Protein Sequence Determination. , 2nd. edn pp 232–279 Edited by Needleman S. B., Berlin Springer-Verlag.
    [Google Scholar]
  6. Fukumori F., Kudo T., Horikoshi K. 1985; Purification and properties of a cellulase from alkalophilic Bacillus sp. no. 1139. Journal of General Microbiology 131:3339–3345
    [Google Scholar]
  7. Gilkes N. R., Langsford M. L., Kilburn D. G., Miller R. C. JR, Warren R. A. J. 1984; Mode of action and substrate specificities of cellulases from cloned bacterial genes. Journal of Biological Chemistry 259:10455–10459
    [Google Scholar]
  8. Hawke D., Yan P. M., Shively J. E. 1982; Microsequence analysis of peptides and proteins. 11. Separation of amino acid phenylthiohydantoin derivatives by high-performance liquid chromatography on octadecylsilane supports. Analytical Biochemistry 120:302–311
    [Google Scholar]
  9. Horikoshi K., Akiba T. 1982; Alkalophilic Microorganisms. New York : Springer-Verlag:
    [Google Scholar]
  10. Horikoshi K., Nakao M., Kurono Y., Sashihara N. 1984; Cellulase of an alkalophilic Bacillus strain isolated from soil. Canadian Journal of Microbiology 30774–779
    [Google Scholar]
  11. Kato C., Kudo T., Watanabe K., Horikoshi K. 1983; Extracellular production of Bacillus penicillinase by Escherichia coli carrying pEAP2. European Journal of Applied Microbiology and Biotechnology 18:339–343
    [Google Scholar]
  12. Kato C., Kudo T., Watanabe K., Horikoshi K. 1985; Nucleotide sequence of the P-lactamase gene of alkalophilic Bacillus sp. strain 170. Journal of General Microbiology 131:3317–3324
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Lederberg E. M., Cohen S. N. 1974; Transformation of Salmonella typhirnurium by plasmid deoxyribonucleic acid. Journal of Bacteriology 119:1072–1074
    [Google Scholar]
  15. Maniatis T., Fritsch E. F., Sambrook J. 1981; Molecular Cloning, a Laboratory Manual. pp 68–69 Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Mclaughlin J. R., Murray C. L., Rabinowitz J. C. 1981; Unique features in the ribosome binding site sequence of the gram-positive Staphylococcus aureus p-lactamase gene. Journal of Biological Chemistry 256:11283–11291
    [Google Scholar]
  17. Messing J. 1983; New M13 vector for cloning. Methods in Enzymology 101:20–78
    [Google Scholar]
  18. Messing J., Crea R., Seeburg P. H. 1981; A system for shotgun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  19. Murphy N., Mcconnell D. J., Cantwell B. A. 1984; The DNA sequence of the gene and genetic control sites for the excreted in Bacillus subtilis enzyme β-glucanase. Nucieic Acids Research 12:5355–5367
    [Google Scholar]
  20. Murray C. L., Rabinowitz J. C. 1982; Nucleotide sequences of transcription and translation regions in Bacillus phage ϕ29 early genes. Journal of Biological Chemistry 257:1053–1062
    [Google Scholar]
  21. Palva I., Pettersson R. F., Kalkkinen N., Lehtovaara P., Sarvas M., Söderlund H., Takkinen K., Kääriäinen L. 1981; Nucleotide sequence of the promoter and NH1-terminal signal peptide region of the α-amylase gene from Bacillus amyloliquejaciens. Gene 15:43–51
    [Google Scholar]
  22. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in civo by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  23. Rosenberg M., Court D. 1979; Regulatory sequences involved in the promotion and termination of RN A transcription. Annual Reuiew ofGenetics
    [Google Scholar]
  24. Saito H., Miura K. 1963; Preparation of transforming DNA by phenol treatment. Biochimica et biophysica acta 72:619–629
    [Google Scholar]
  25. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings ojthe National Academy of Sciences ofthe United States of America 74:5463–5467
    [Google Scholar]
  26. Sashihara N., Kudo T., Horikoshi K. 1984; Molecular cloning and expression of cellulase genes of alkalophilic Bacillus sp. strain N-4 in Escherichia coli. Journal of Bacteriology 158:503–506
    [Google Scholar]
  27. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  28. Stephens M. A., Ortlepp S. A., Ollington J. F., Mcconnel D. J. 1984; Nucleotide sequence of the 5′ region of the Bacillus lichenijormis α-amylase gene : comparison with the B. amyloliquejaciens gene. Journal of Bacteriology 158:369–372
    [Google Scholar]
  29. Tinoco I. JR, Borer P. N., Dengler B., Levine M. D., Uhlenberk O. C., Crothers D. M., Gralla J. 1973; Improved estimation of secondary structure in ribonucleic acids. Nature New Biology 246:40–41
    [Google Scholar]
  30. Yang M., Galizzi A., Henner D. 1983; Nucleotide sequence of the amylase gene from Bacillus subtilis. Nucleic Acids Research 11:237–249
    [Google Scholar]
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