1887

Abstract

Summary: A soil isolate, 4B, which had been previously assigned to the genus and shown to be capable of reducing CH with simple phenolic compounds as sole carbon source, was further characterized in comparison with two other diazotrophs which were identified as pseudomonads. The DNA base composition of 4B was 60·2 mol% G + C. Plasmid DNA was not detected in alkaline SDS lysates of 4B by agarose gel electrophoresis. Comparable maximum CH reduction activities in 4B were observed under microaerobic conditions (pO about 0·003 atm) with either 28 mM-glucose or 5 mM-protocatechuate as carbon source. N fixation was confirmed by the cellular incorporation of N in cultures of 4B grown in N-free medium. Extensive biochemical tests, including the carbon utilization pattern, demonstrated that 4B was closely related to (ATCC 17505) although the latter did not fix N. 4B had metabolic patterns different from the two other strains reported to be diazotrophic pseudomonads; all three contained DNA homologous to the genes of M5A1.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-8-2277
1986-08-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/8/mic-132-8-2277.html?itemId=/content/journal/micro/10.1099/00221287-132-8-2277&mimeType=html&fmt=ahah

References

  1. Balandreau J. 1983; Microbiology of the association. Canadian Journal of Microbiology 29:851–859
    [Google Scholar]
  2. Barraquio W. L., Ladha J. K., Watanabe I. 1983; Isolation and identification of N2-fixing Pseudomonas associated with wetland rice. CanadianJournal of Microbiology 29:867–873
    [Google Scholar]
  3. Barraquio W. L., Padre B. C. JR, Watanabe I., Knowles R. 1986; Nitrogen fixation by Pseudo monas saccharophila Doudoroff ATCC 15946. Journal of General Microbiology 132:237–241
    [Google Scholar]
  4. Botchan M., Topp W., Sambrook J. 1976; The arrangement of Simian virus 40 sequences in the DNA of transformed cells. Cell 9:269–287
    [Google Scholar]
  5. Brill W. J. 1980; Biochemical genetics of nitrogen fixation. Microbiological Reviews 44:449–467
    [Google Scholar]
  6. Cannon F. C., Riedel G. E., Ausubel F. M. 1979; Overlapping sequences of Klebsiella pneumoniae nif DNA cloned and characterized. Molecular and General Genetics 174:59–66
    [Google Scholar]
  7. Chan Y.-K. 1985; Denitrification by a diazotrophic Pseudomonas species. Canadian Journal of Microbiology 31:1136–1141
    [Google Scholar]
  8. Chan Y.-K. 1986; Utilization of simple phenolics for dinitrogen fixation by soil diazotrophic bacteria. Plant and Soil 90:141–150
    [Google Scholar]
  9. Dalton H. 1980; The cultivation of diazotrophic microorganisms. In Methods of Evaluating Biological Nitrogen Fixation pp. 13–62 Edited by Bergersen F. J. New York: John Wiley;
    [Google Scholar]
  10. Davis D. H., Stanier R. Y., Doudorofp M. 1970; Taxonomic studies on some Gram negative polarly flagellated “hydrogen bacteria” and related species. Archives of Microbiology 70:1–13
    [Google Scholar]
  11. De Bont J. A. M., Leijten M. W. M. 1976; Nitrogen fixation by hydrogen-utilizing bacteria. Archives of Microbiowgy 107:235–240
    [Google Scholar]
  12. Deley J., De Vos P. 1984; The genus Pseudomonas. Antonie van Leeuwenhoek 50:281–283
    [Google Scholar]
  13. Deley J., Park I. W. 1966; Molecular biological taxonomy of some free-living nitrogen-fixing bacteria. Antonie van.Leuwenhoek 32:6–16
    [Google Scholar]
  14. Derylo M., Glowacka M., Sicorupska A., Loriciewicz Z. 1981; Nif plasmid from Ligno bacter. Archives of Microbiology 130:322–324
    [Google Scholar]
  15. De Smedt J., Bauwens M., Tytoat R., Deley J. 1980; Intra-and intergeneric similarities of riboso mal ribonucleic acid cistrons of free-living, nitrogen fixing bacteria. International Journal of Systematic Bacteriology 30:l06–122
    [Google Scholar]
  16. De Vos P., De Ley J. 1983; Intra-and intergeneric similarities of Pseudomonas and Xanthomonas riboso mal ribonucleic acid cistrons. lntemational Journal of Systematic Bacteriology 33:487–509
    [Google Scholar]
  17. Eckhardt T. 1978; A rapid method for the identification of plasmid deoxyribonucleic acid in bacteria. Plasmid l:584–588
    [Google Scholar]
  18. Elmerich C. 1984; Molecular biology and ecology of diazotrophs associated with non-leguminous plants. Biotechnology 2:967–978
    [Google Scholar]
  19. Guerry P., Leblanc D. J., Falicow S. 1973; General method for the isolation of plasmid deoxy ribonucleic acid. Journal of Bacteriology 116:1064–1066
    [Google Scholar]
  20. Haahtela K., Helander I., Nurmiaho-lassila E-L., Sundman V. 1983; Morphological and physiological characteristics and lipopolysaccharide composition of N2-fixing (C2H2-reducing) root associated Pseudomonas sp. Canadian Journal of Microbiology 29:874–880
    [Google Scholar]
  21. Hill s., Postoate J. R. 1969; Failure of putative nitrogen-fixing bacteria to fix nitrogen. Journal of General Microbiowgy 58:277–285
    [Google Scholar]
  22. Hugh R., Leipson E. 1953; The taxonomic significance of fermentative versus oxidative meta bolism of carbohydrates by various Gram-negative bacteria. Journal of Bacteriology 66:24–26
    [Google Scholar]
  23. Keeney D. R., Nelson D. W. 1982; Nitrogen inorganic forms. In Methods of Soil Analysis part 2 pp. 643–698 Edited by Page A. L., Miller R. H., Keeney D. R. Madison: American Society of Agronomy;
    [Google Scholar]
  24. Knowles R. 1977; The significance of asymbiotic dinitrogen fixation by bacteria. In A Treatise on Dinitrogen Fixation. Section IV: Agronomy and Ecology pp. 33–83 Edited by Hardy R. W. F., Gibson A. H. New York: John Wiley;
    [Google Scholar]
  25. Knowles R. 1978; Free-living bacteria. In Limitations and Potentials for Biological Nitrogen Fixation in the Tropics pp. 25–40 Edited by Dobereiner J., Burris R. H., Hollaender A. New York: Plenum Press;
    [Google Scholar]
  26. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from microorganisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  27. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  28. Murray K., Duooleby C. J., Sala-trepat J. M., Williams P. A. 1972; The metabolism of benzoate and methylbenzoate via the meta-cleavage pathway of Pseudomonas arvilla mt-2. European Journal of Biochemistry 28:301–310
    [Google Scholar]
  29. Palleroni N. J. 1984; Genus I Pseudomonas Migula 1984. In Bergey's Manual of Systematic Bacteriology, vol 1 pp. 141–199 Edited by Krieg N. R., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  30. Postgate J. R. 1981; Microbiology of the free-living nitrogen-fixing bacteria, excluding cyanobacteria. In Current Perspectives in Nitrogen Fixation pp. 217–228 Edited by Gibson A. H., Newton W. E. Canberra: Australian Academy of Science;
    [Google Scholar]
  31. Postgate J. R. 1982; Biological nitrogen fixation: fundamentals. Philosophical Transactions of the Royal Society of London, Series B 296:375–385
    [Google Scholar]
  32. Preston C. M., Preston J. M., Callway E. G. 1981; Inexpensive 15N analysis of agricultural samples by optical emission spectroscopy employing a simple, one-step Dumas sample preparation procedure. Canadian Journal of Spectroscopy 26:239–244
    [Google Scholar]
  33. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  34. Robson R., Postoate J. 1980; Oxygen and hydrogen in biological nitrogen fixation. Annual Review of Microbiology 34:183–207
    [Google Scholar]
  35. Robson R., Kennedy C., Postoate J. R. 1983; Progress in comparative genetics of nitrogen fixation. Canadian Journal of Microbiology 29:954–967
    [Google Scholar]
  36. Ruvkun G. B., Ausubbl F. M. 1980; Interspecies homology of nitrogenase genes. Proceedings of the National Academy of Sciences of the United States of America 77:191–195
    [Google Scholar]
  37. Singh M., Kleeberger A., Klinmullbll W. 1983; Location of nitrogen fixation (nif) genes on indigenous plasmids of Enterobacter agglomerans. Molecular and General Genetics 190:373–378
    [Google Scholar]
  38. Southern E. M. 1975; Detection of specific se quences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  39. Stanier R. Y., Pallbroni N. J., Doudoroff M. 1966; The aerobic pseudomonads: a taxonomic study. Journal of General Microbiology 43:159–271
    [Google Scholar]
  40. Ulitzur S. 1972; Rapid determination of DNA base composition by ultraviolet spectroscopy. Biochimica et biophysica acta 272:1–1l
    [Google Scholar]
  41. Wheatcroft R., Williams P. A. 1981; Rapidmethods for the study of both stable and unstable plasmids in Pseudomonas. Journal of General Microbiology 124:433–437
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-8-2277
Loading
/content/journal/micro/10.1099/00221287-132-8-2277
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error