1887

Abstract

Recombinant plasmids encoding a haemagglutinin were isolated from the highly virulent strain C5 by cosmid cloning. Both HB101 containing the recombinant plasmids and C5 were able to agglutinate a variety of erythrocytes from human and animal origin; this haemagglutination was not inhibited by D-mannose or L-fucose. Subcloning of the recombinant cosmid DNA revealed that a 1.3 kb DNA fragment was sufficient for haemagglutinin production in HB101. Under direction of this 1.3 kb DNA fragment, two proteins were made in minicells, of 27 and 10 kDa. Haemagglutinin-encoding sequences were not detected in every strain.

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/content/journal/micro/10.1099/00221287-132-8-2225
1986-08-01
2019-10-14
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-8-2225
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