Recombinant plasmids encoding a haemagglutinin were isolated from the highly virulent strain C5 by cosmid cloning. Both HB101 containing the recombinant plasmids and C5 were able to agglutinate a variety of erythrocytes from human and animal origin; this haemagglutination was not inhibited by D-mannose or L-fucose. Subcloning of the recombinant cosmid DNA revealed that a 1.3 kb DNA fragment was sufficient for haemagglutinin production in HB101. Under direction of this 1.3 kb DNA fragment, two proteins were made in minicells, of 27 and 10 kDa. Haemagglutinin-encoding sequences were not detected in every strain.


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