1887

Abstract

Summary: The wide spectrum amidase from sp. R312, which can hydrolyse many amides tothe corresponding acids, was shown to transfer the acyl groups of amides, acids and esters to hydroxylamine. The transfer rates ofthese reactions in cytoplasmic fractions were measured and compared. The and were determined for different substrates in the presence of hydroxylamine. The enzyme was also shown to transfer the acyl group of the amide analogue N–methylacetamide to hydroxylamide and that of acetamide to the hydroxylamine analogue methylhydroxylamine.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-8-2205
1986-08-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/8/mic-132-8-2205.html?itemId=/content/journal/micro/10.1099/00221287-132-8-2205&mimeType=html&fmt=ahah

References

  1. Arnaud A., Galzy P., Jallageas J. C. 1976a; Remarques sur l'activite nitrilasique de quelques bacteries. Comptes rendus de l'Academie des Sciences 283:571–573
    [Google Scholar]
  2. Arnaud A., Galzy P., Jallageas J. C. 1976b; Etude de l'activite nitrilasique de quelques bacteries. Revue des fermentations et des industries alimentaires 31:39–44
    [Google Scholar]
  3. Arnaud A., Galzy P., Jallageas J. C. 1977; Etude de l'acetonitrilase d'une souche de Brevibacterium. Agricultural and Biological Chemistry 41:2183–2191
    [Google Scholar]
  4. Asano Y., Tachibana M., Tani Y., Yamada H. 1982; Purification and characterization of amidase which participates in nitrile degradation. Agricultural and Biological Chemistry 46:1175–1181
    [Google Scholar]
  5. Brammar W. J., Clarke P.H. 1964; Induction and repression of Pseudomonas aeruginosa amidase. Journal of General Microbiology 37:307–319
    [Google Scholar]
  6. Bui K., Fradet H., Arnaud A., Galzy P. l984a; A nitrile hydratase with a wide substrate spectrum produced by a Brevibacterium sp. Journal of General Microbiology 130:89–93
    [Google Scholar]
  7. Bui K., Fradet H., Thièry A., Maestracci M., Arnaud A., Galzy P. 1984b; Use of NMR and GLC for the study of microbial nitrile hydratases and amidases. Analytica chimica acta 163:315–322
    [Google Scholar]
  8. Clarke P. H. 1970; The aliphatic amidases of Pseudomonas aeruginosa. Advances in Microbial Physiology 4:179–222
    [Google Scholar]
  9. Jallageas J. C., Arnaud A., Galzy P. 1978a; Etude de l'acetamidase d'une souche de Brevibacterium. Journal of General and Applied Microbiology 24:103–114
    [Google Scholar]
  10. Jallageas J. C., Arnaud A., Galzy P. 1978b; Application de la chromatographie en phase gazeuse a l'etude des nitrilases et des amidases. Journal of Chromatography 166:181–187
    [Google Scholar]
  11. Maestracci M., Thièry A., Bui K., Arnaud A., Galzy P. 1984; Activity and regulation of an amidase (acylamide amidohydrolase EC 3.5. l.4) with a wide substrate spectrum from a Brevibacterium sp. Archives of Microbiology 138:315–320
    [Google Scholar]
  12. Miller J. M., Gray D. O. 1982; The utilization and amides by a Rhodococcus sp. Journal of General Microbiology 128:1803–1809
    [Google Scholar]
  13. Thalenfeld B., Grossowicz N. 1976; Regulatory properties of an inducible aliphatic amidase from a thermophilic Bacillus. Journal of General Microbiology 94:131–141
    [Google Scholar]
  14. ThiÉry A., Maestracci M., Arnaud A., Galzy P. 1986a; Nitriles as growth substrates for Brevi bacterium sp. R 312 and its mutant M2. Zentralblatt for Mikrobiologie in the Press
    [Google Scholar]
  15. ThiÉry A., Maestracci M., Arnaud A., Galzy P., Nicolas M. 1986b; Purification and properties of an acylamide amidohydrolase (EC 3. 5. 1, 4) with a wide activity spectrum from Brevibacterium sp. R312. Journal of Basic Microbiology (in the Press)
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-8-2205
Loading
/content/journal/micro/10.1099/00221287-132-8-2205
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error