The haem requirement for growth of the rumen chytridiomycete H8 was satisfied by haem, haemin, haematin, mesoporphyrin IX, coproporphyrins I and III, uroporphyrins I and III, but not deuteroporphyrin IX. Porphyrin degradation products and precursors of porphyrin synthesis did not support growth. Protoporphyrin IX and haemato-porphyrin IX supported growth, indicating that the organism contains ferrochetalase, but was unable to synthesize the porphyrin ring. Haem could be provided by catalase and peroxidase, but not by cytochrome Zoosporogenesis was induced in H8 in rumen fluid principally by haem, haemin and haematin, and to a smaller extent by catalase, peroxidase and protoporphyrin IX. Haem precursors and degradation products were ineffective. Chlorophylls and and their ruminal degradation products, plant fraction 1 protein and chelated iron neither stimulated growth nor induced zoosporogenesis. Haem induced partial zoosporogenesis in haem-limited cultures and suspensions of sporangia grown


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error