RT Journal Article SR Electronic(1) A1 Parkin, Michael J. A1 Ross, I. StuartYR 1986 T1 The Specific Uptake of Manganese in the YeastCandida utilis JF Microbiology, VO 132 IS 8 SP 2155 OP 2160 DO https://doi.org/10.1099/00221287-132-8-2155 PB Microbiology Society, SN 1465-2080, AB Summary: Uptake of 54Mn from 10 nM-Mn2+ was shown to be both energy-and pH-dependent. Analysis of the uptake kinetics revealed an apparent half-saturation constant, K t of 16·4 nM-Mn2+ and a maximal rate of transport, V max,of l·01 nmol Mn2+ (g dry wt)−1 min−1 Mn2+ uptake was highly specific, being unaffected by 100-fold molar excess of Mg2+, zn2+, Ca2+, Co2+, Ni2+ and Cu2+; however, uptake was inhibited 30 to 40% by 1000-fold molar excess of Mg2+, Zn2+, Ca2+, Co2+ and Ni2+. Zn2+ competitively inhibited Mn2+ uptake, theK i value being approximately 500-fold greater than the Kt for Mn2+. Efflux studies indicated that metabolic exchange of 54Mn occurred to a small extent. Cellular Mn2+ contents remained relatively constant during growth in batch culture. The Mn2+ transport system observed appears to be analogous to the specific metal transport systems reported in bacteria., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-8-2155