1887

Abstract

Summary: L-Lysine transport in was investigated. The bacterium was shown to possess a highly specific, energy-dependent system of active lysine transport. The system transferred lysine into the cells and exchanged intra-and extracellular lysine. Mutations in the transport system did not lead to overproduction ofthe amino acid. Resting cells ofthe parent strain, or of its lysine-producer derivatives with a defective transport system, failed to excrete lysine into the medium. An efflux ofintracellular lysine could be induced by a hyperosmotic shock, and by different membrane-active substances. It has been suggested that cells are equipped with channels (pores) for excreting lysine from the cytoplasm. These channels appeared to open in response to an increase in the intracellular lysine concentration. The channel permeability also depends on the membrane structure.

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/content/journal/micro/10.1099/00221287-132-8-2137
1986-08-01
2021-08-05
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References

  1. Boos W. 1974; Bacterial transport. Annual Review of Biochemistry 43:123–146
    [Google Scholar]
  2. Broda P. 1968; Ribonucleic acid synthesis and glutamate excretion in Escherichia coli. Journal of Bacteriology 96:1528–1534
    [Google Scholar]
  3. Clement Y., Escoffier B., Trombe M. C., Laneele J. 1984; Is glutamate excreted by its uptake system in Corynebacterium glutamicum? A working hypothesis. Journal ofGeneral Microbiology 130:2589–2594
    [Google Scholar]
  4. Demain A. L., Birnbaum J. 1968; Alteration of permeability for the release of metabolites from the microbial cell. Current Topics in Microbiology 46:1–26
    [Google Scholar]
  5. Foster T. J. 1983; Plasmid-determined resistance to antimicrobial drug and toxic metal ions in bacteria. Microbiological Reviews 47:361–409
    [Google Scholar]
  6. Furuya A., Misawa M., Nara T., Abe S., Kinoshita S. 1969; Metabolic controls of accumulation of aminoacids and nucleotides. In Fermentation Advances pp. 177–198 Edited by Perlman D. New York & London: Academic Press;
    [Google Scholar]
  7. Goldenbaum P. E., Hall G. A. 1979; Transport of cyclic adenosine-3', 5'-monophosphate across Escherichia coli vesicle membranes. Journal of Bacteriology 140:459–467
    [Google Scholar]
  8. Griffin D. M. 1981; Water and microbial stress. Advances in Microbial Ecology 5:137–210
    [Google Scholar]
  9. Halsall D. M. 1975; Overproduction of lysine by mutant strains of Escherichia coli with defective lysine transport system. Biochemical Genetics 13:109–124
    [Google Scholar]
  10. Hamilton W. A. 1975; Energy coupling in microbial transport. Advances in Microbial Physiology 12:1–53
    [Google Scholar]
  11. Hänel F., Hillinger M., Gräfe U. 1981; Effect of oxygen limitation on cellular L-lysine pool and lipid spectrum in Corynebacterium glutamicum. Biotechnology Letters 3:461–464
    [Google Scholar]
  12. Kara-Murza S. N., Ivanovskaya L. V., Zhdanova N. I. 1978; Effect of amino acids on β-aspartokinase activity of a wild type Corynhacterium glutamicum strain and its mutant. Prikladnaya biokhimia i mikrobiologia 14:345–349 (in Russian)
    [Google Scholar]
  13. Kinoshita S., Nakayama K., Kitaga S. 1958; L-Lysine production using microbial auxotroph. Journal of General and Applied Microbiology. 4:128–129
    [Google Scholar]
  14. Leonova T. V., Ivanovskaya L. V., Zhdanova N. I., Kara-Murza N. 1981; Some properties of analogue-resistant mutants of C. glutamicum pro-ducing L-lysine. In Abstract Book of the FEMS Symposium on Overproduction of Microbial Products pp. 212–213 Czechoslovakia: Hradec Kralove;
    [Google Scholar]
  15. Nakayama K., Kumagai H., Tochikura T. 1984; Leakage of glutathione from bacterial cells caused by inhibition of γ-glutamyltranspeptidase. Applied and Environmental Microbiology 47:653–657
    [Google Scholar]
  16. Niven D. F., Hamilton A. 1973; Valinomy cin-induced amino acid uptake by Staphylococcus aureus. FEBS Letters 37:244–248
    [Google Scholar]
  17. Niven D. F., Hamilton A. 1974; Mechanisms of energy coupling to the transport of amino acids by Staphylococcus aureus . European Journal of Biochemistry 44:517–522
    [Google Scholar]
  18. Ozaki H., Shiio J. 1983; Production of lysine by pyruvate kinase mutants of Brevibacterium falauum. Agricultural and Biological Chemistry 47:1569–1576
    [Google Scholar]
  19. Ramos s., Kaback H. R. 1977; The relationship between the electrochemical proton gradient and active transport in Escherichia coli membrane vesicles. Biochemistry 16:854–859
    [Google Scholar]
  20. Robertson D. E., Kaczorowski H. J., Garcia M.-L., Kaback H. R. 1980; Active transport in membrane vesicles from Escheri-chia coli: the electrochemical proton gradient alters the di-stribution of the lac carrier between two different kinetic states. Biochemistry 19:5692–5702
    [Google Scholar]
  21. Rosen B. P., Mcclees J. 1974; Active transport of calcium in inverted membrane vesicles of Escherichia coli. Proceedings of the National Academy of Sciences of the United States of America 71:5042–5046
    [Google Scholar]
  22. Sibirny A. A., Shavlovsky G. M., Ksheninskaya G. P., Orlovsky A. G. 1978; Effect of glucose and its derivatives on the systems of absorption and excretion of riboflavin in yeast Pichia guilliermondii. Biokhimiya 43:1414–1422 (in Russian)
    [Google Scholar]
  23. Teshiba S., Furuya A. 1983; Mechanisms of 5’-inosinic acid accumulation by permability mutants of Brevibacterium ammoniagenes. III. Intracellular 5’-IMP pool and excretion mechanisms of 5'-IMP. Agricultural and Biological Chemistry 47:2357–2363
    [Google Scholar]
  24. Zahorchak R. J., Winkler H. H. 1983; Trans-membrane electrical potential in Rickettsia prowazekii and its relationship to lysine transport. Journal Bacteriology 153:665–671
    [Google Scholar]
  25. Zaitseva M., Chernysheva I. P. 1975; Some properties of β-aspartokinase from Micmcoccus glutamicus-95 . Biokhimiya 40:71–75 (in Russian)
    [Google Scholar]
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