L-Lysine transport in was investigated. The bacterium was shown to possess a highly specific, energy-dependent system of active lysine transport. The system transferred lysine into the cells and exchanged intra- and extracellular lysine. Mutations in the transport system did not lead to overproduction of the amino acid. Resting cells of the parent strain, or of its lysine-producer derivatives with a defective transport system, failed to excrete lysine into the medium. An efflux of intracellular lysine could be induced by a hyperosmotic shock, and by different membrane-active substances. It has been suggested that cells are equipped with channels (pores) for excreting lysine from the cytoplasm. These channels appeared to open in response to an increase in the intracellular lysine concentration. The channel permeability also depends on the membrane structure.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error