1887

Abstract

Summary: secretes five isoenzymes of pectate lyase (PLa to PLe), which are involved in the phytopathogenicity of this bacterial species. We describe the isolation and characterization of mutants in the regulatory systems controlling PL synthesis. The regulation of PL synthesis appears to be complex, involving several controls. In one class of mutants, designated , the synthesis of various proteins became resistant to catabolite repression. This mutation mainly affected the synthesis of isoenzymes PLb and PLc. In a second class of mutants, designated , PL synthesis was no longer inducible in the presence of pectin derivatives; the regulatory gene responsible for the induction was evidently inactivated. Moreover mutations led to synthesis of PL independent of the growth phase, whereas in the wild-type strain PL synthesis increased only at the end of exponential growth. These mutations mainly affected the synthesis of PLa, PLd and PLe. Mutations in , the regulatory gene controlling 2-keto-3-deoxygluconate catabolism, had the same effects on PL synthesis as mutations.

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1986-08-01
2022-01-25
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References

  1. Andro T., Chambost J., Kotoujansky A., Cattaneo J., Bertheau Y., Barras F., Van Gijsegem F., Coleno A. 1984; Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase. Journal of Bacteriology 160:1199–1203
    [Google Scholar]
  2. Bashman H., Bateman D. 1975; Relationship of cell death in plant tissue treated with a homogeneous endopectate lyase to cell wall degradation. Physiological Plant Pathology 5:249–262
    [Google Scholar]
  3. Bertheau Y., Madgidi-Hervan E., Kotoujansky A., Njuyen-The C., Andro T., Coleno A. 1984; Detection of depolymerase isoenzymes after electrofocusing or in titration curves. Analytical Biochemistry 139:383–389
    [Google Scholar]
  4. Chatterjee A., Starr M. 1980; Genetics of Erwinia species. Annual Review of Microbiology 34:645–676
    [Google Scholar]
  5. Collmer A., Bateman D. 1981; Impaired induction and self-catabolite repression of extracellular pectate lyase in Erwinia chrysanthemi mutants deficient in oligo-galacturonide lyase. Proceedings of the National Academy of Sciences of the United States of America 78:3920–3924
    [Google Scholar]
  6. Condemine G., Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. 1984; An enzyme in the pectinolytic pathway of Erwinia chrysanthemi: 2-keto-3-deoxygluconate oxidoreductase. Journal of General Microbiology 130:2839–2844
    [Google Scholar]
  7. Condemine G., Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. 1986; Isolation of Erwinia chrysanthemi kduD mutants altered in pectin degradation. Journal of Bacteriology 165:937–941
    [Google Scholar]
  8. De Crombrugghe B., Busby S., Buc H. 1984; Cyclic AMP receptor protein: role in transcription activation. Science 224:831–838
    [Google Scholar]
  9. Hamon Y., Peron Y. 1961; Les proprietes antagonistes reciproques parmi les Erwinia. Discussion de la position taxonomique de ce genre. Comptes rendus des seances de I’Academie des sciences 235:913–915
    [Google Scholar]
  10. Hubbard J., Williams J., Niles R., Mount M. 1978; The relation between glucose repression and endo-polygalacturonate trans-eliminase and adenosine 3' :5'-cyclic monophosphate levels. in Erwinia carotovora Phytopathology 68:95–99
    [Google Scholar]
  11. Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. 1985a; Isolation of kdgK-lac and kdgA-lac gene fusions in the phytopathogenic bacterium Erwinia chrysanthemi. Journal of General Microbiology 131:1205–1211
    [Google Scholar]
  12. Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. 1985b; Lactose metabolism in Erwinia chrysanthemi. Journal of Bacteriology 162:248–255
    [Google Scholar]
  13. Hugouvieux-Cotte-Pattat N., Quesneau Y., Robert-Baudouy J. 1983; Aldohexuronate transport system in Erwinia carotovora. Journal of Bacteriology 154:663–668
    [Google Scholar]
  14. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor NY:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  15. Moran F., Starr M. 1969; Metabolic regulation of polygalacturonic acid trans-eliminase in Erwinia. European Journal of Biochemistry 11:291–295
    [Google Scholar]
  16. Moran F., Nasuno S., Starr M. 1968; Extracellular and intracellular polygalacturonic acid transeliminase of Erwinia carotovora. Archives of Biochemistry and Biophysics 123:298–306
    [Google Scholar]
  17. Reverchon S., Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. 1985; Cloning of genes encoding pectolytic enzymes from a genomic library of the phytopathogenic bacterium, Erwinia chrysanthemi. Gene 35:121–130
    [Google Scholar]
  18. Schoonejans E., Toussaint A. 1983; Utilization of plasmid pULB 113 (RP4:: mini-Mu) to construct a linkage map of Erwinia carotovora subsp. chrysanthemi. Journal of Bacteriology 154:1489–1492
    [Google Scholar]
  19. Starr M., Chatterjee A. 1972; The genus Erwinia: enterobacteria pathogenic to plants and animals. Annual Review of Microbiology 26:389–426
    [Google Scholar]
  20. Ullman A., Danchin A. 1983; Role of cyclic AMP in bacteria. Advances in Cyclic Nucleotide Research 15:1–53
    [Google Scholar]
  21. Van Gijsegem F., Toussaint A. 1982; Chromosome transfer and R' formation by an RP4::mini-Mu derivative in Escherichia coli, Salmonella typhimurium, Klebsiella pneumoniae and Proteus mirabilis. Plasmid 7:30–44
    [Google Scholar]
  22. Van Gijsegem F., Toussaint A. 1983; In vivo cloning of Erwinia carotovoragenes involved in the catabolism of hexuronates. Journal of Bacteriology 154:1227–1235
    [Google Scholar]
  23. Van Gijsegem F., Hugouvieux-Cotte-Pattat N., Robert-Baudouy J. 1985a; Isolation and characterization of Erwinia chrysanthemi mutants defective in degradation of hexuronates. Journal of Bacteriology 161:702–708
    [Google Scholar]
  24. Van Gijsegem F., Toussaint A., Schoonejans E. 1985b; In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi. EMBO Journal 4:787–792
    [Google Scholar]
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