1887

Abstract

Summary: The isolation of different classes of antibiotic-supersensitive outer membrane permeability mutants of has been described previously ( Sukupolvi , 1984 , ,704-712). One of these, the SS-A mutation, sensitizes the bacteria to gentian violet and to hydrophobic antibiotics. The phenotype of the SS-A mutant was restored to normal when a cloned fragment of the F plasmid, or the R plasmid R6-5, carrying the genes , and was introduced on a multicopy plasmid. The introduction of a plasmid carrying only the T gene showed thatthis gene was sufficient to restore the phenotype. Only clones with functioning (irrespective of copy number) restored the normal antibiotic-resistant phenotype in the SS-A mutant. An incompatibility test using a donor strain which carried transposon in the 60 MDa plasmid of and a recipient in which Tn was placed close to the SS-A mutation indicated that the SS-A mutation was located in the 60 MDa virulence plasmid (previously called the cryptic plasmid) of . The introduction of the large virulence plasmid carrying the SS-A mutant allele into wild-type or resulted in strains with a phenotype identical tothat of the original SS-A mutant.

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1986-08-01
2024-03-29
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References

  1. Achtman M., Kennedy N., Skurray R. 1977; Cell-cell interactions in conjugating Escherichia coli: role of traT protein in surface exclusion. Proceedings of the NationalAcademy of Sciences of the United States of America 14:5104–5108
    [Google Scholar]
  2. Achtman M., Kusecek B., Timmis K. N. 1978a; tra cistrons and proteins encoded by the Escherichia coli antibiotic resistance plasmid R6-5. Molecular and General Genetics 163:169–179
    [Google Scholar]
  3. Achtman M., Skurray R. A., Thompson R., Helmuth R., Hall S. L., Beutin L., Clark A. K. 1978b; Assignment of tra cistrons to EcoRI fragments of F sex factor DNA. Journal of Bacteriology 133:1383–1392
    [Google Scholar]
  4. Achtman M., Mannino P. A., Edblblutii C., Herrlich P. 1979; Export without proteolytic processing of inner and outer membrane proteins encoded by F sex factor tra cistrons in Escherichia coli minicells. Proceedings of the National Academy ofSciences of the United States of America 76:4837–4841
    [Google Scholar]
  5. Achtman M., Manning P. A., Kusbcbk B., Schwuchow S., Willetts A. 1980; A genetic analysis of F sex factor cistrons needed for surface exclusion in Escherichia coli . Journal of Molecular Biology 138:779–795
    [Google Scholar]
  6. Agüero M. E., Aron L., Deluca A. G., Timmis K. N., Cabello F. C. 1984; A plasmid-encoded outer membrane protein, TraT, enhances resistance of Escherichia coli to phagocytosis. Infection and Immunity 46:740–746
    [Google Scholar]
  7. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  8. Chang A. C. Y., Cohen S. N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the pl5A cryptic miniplasmid. Journal of Bacteriology 134:1141–1156
    [Google Scholar]
  9. Cohen S. N., Chang A. C. Y., Boyer H. W., Helling R. B. 1973; Construction of biologically functional plasmids in vitro . Proceedings of the National Academy of Sciences of the United States of America 70:3240–3244
    [Google Scholar]
  10. Coleman w., Leive L. 1979; Two mutations which affect the barrier function of the Escherichia coli K-12 outer membrane. Journal of Bacteriology 139:899–910
    [Google Scholar]
  11. Edwards M. 1985; Theft+ plasmid of Salmonella typhimurium. PhD thesis Stanford University; Calif., USA;
    [Google Scholar]
  12. Jones G. W., Robert D. K., Svinarich D. M., Whitfield H. J. 1982; Association of adhesive, invasive and virulent phenotypes of Salmonella typhimurium with autonomous 60-megadalton plas mids. Infection and Immunity 38:476–486
    [Google Scholar]
  13. Kennedy N., Beutin L., Achtman M., Skurray R., Rahmsoorf u., Herrlich P. 1977; Conjugation proteins encoded by the F sex factor. Nature, London 270:580–585
    [Google Scholar]
  14. Mäkelä P. H., Lederberg J., Lederberg E. M. 1962; Patterns of sexual recombination in enteric bacteria. Genetics 47:1427–1439
    [Google Scholar]
  15. Mandel M., Higa A. 1970; Calcium-dependent bacteriophage DNA infection. Journal of Molecular Biology 53:159–162
    [Google Scholar]
  16. Maniatis T., Fritsch E. F., Sambrook J. 1982; Molecular Cloning, a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Manning P. A., Beutin L., Achtman M. 1980; Outer membrane of E. coli: properties of the F sex factor traT protein which is involved in surface exclusion. Journal of Bacteriology 142:285–294
    [Google Scholar]
  18. Manning P. A., Timmis J. K., Moll A., Timmis K. N. 1982; Mutants that overproduce TraTp, a plasmid-specified outer membrane protein of Escherichia coli. Molecular and General Genetics 187:426–431
    [Google Scholar]
  19. Moll A., Manning P. A., TimmiS K. N. 1980; Plasmid-determined resistance to serum bactericidal activity: a major outer membrane protein, the traT gene product is responsible for plasmid-specified serum resistance in Escherichia coli . Infection and Immunity 28:359–367
    [Google Scholar]
  20. Montenegro M. A., Bmer-Subrmann D., Timmis J. K., Agüero M. E., Cabello F., Sanyal S. C., Timmis K. N. 1985; traT gene sequences, serum resistance and pathogenicity-related factors in clini cal isolates of Escherichia coli and other Gram negative bacteria. Journal of General Microbiology 131:1511–1521
    [Google Scholar]
  21. Palva E. T., Mäkelä P. H. 1980; Lipopolysac charide heterogeneity in Salmonella typhimurium analyzed bysodium dodecyl sulfate polyacrylamide gel electrophoresis. European Journal of Biochemistry 107:137–143
    [Google Scholar]
  22. Perumal N. B., Minkley E. G. JR 1984; The product of the F sex factor traT surface exclusion gene is a lipoprotein. Journal of Biological Chemistry 259:5357–5360
    [Google Scholar]
  23. Sanderson K. E., Ross H., Ziegler L., Mäkelä P. H. 1972; Hfr and F’ strains of Salmonella typhimurium and Salmonella abony . Bacteriological Reviews 36:608–637
    [Google Scholar]
  24. Sharp P. A., Sugden B., Sambrook J. 1973; Detection of two restriction endonuclease activities in Haemophilus influenzae using analytical agarose ethidium bromide electrophoresis. Biochemistry 12:3055–3063
    [Google Scholar]
  25. Skurray R., Nagaishi H., Clark A. J. 1976; Molecular cloning of DNA from F sex factor of Escherichia coli K-12. Proceedings of the National Academy of Sciencesof the United States of America 73:64–68
    [Google Scholar]
  26. Smith H. R., Humphreys G. O., Grindley N. D. F., Grindley J. N., Anderson E. S. 1973; Molecular studies of an fi+ plasmid from strains of Salmonella typhimurium. Molecular and General Genetics 126:143–151
    [Google Scholar]
  27. Sukupolvi S., Vaara M., Helander I. M., Viljanen P., Mäkelä P. 1984; New Salmonella typhimurium mutants with altered outer membrane permeability. Journal of Bacteriology 159:704–712
    [Google Scholar]
  28. Vaara M., Viuanen P., Sukupolvi S., Vaara T. 1985; Does polymyxin B nonapeptide increase outer membrane permeability in antibiotic supersen sitive enterobacterial mutants?. FEMS Microbiology Letters 26:289–294
    [Google Scholar]
  29. Vieira I., Messing I. 1982; The pUC plasmids, an Ml3mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268
    [Google Scholar]
  30. Willetts N., Skurray R. 1980; The conjugation system of F-like plasmids. Annual Review of Genetics 14:41–76
    [Google Scholar]
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