1887

Abstract

Summary: The 10·8 kb plasmid pJVl, isolated from , has a high copy number (about 150) and a broad host range among spp. Several pJVl derivatives carrying the thiostrepton resistance gene () of were made. One derivative, pWOR191, was shown to promote its own transfer and to mobilize chromosomal markers in Another derivative, pWOR109, was non-transmissible. Deletion of a segment of pWOR109 gave pWOR120 (5·6 kb), which has single HI and II sites shown to be capable of accepting ‘foreign’ DNA such as a previously cloned DNA fragment encoding tyrosinase, giving vectors (pWOR125, pWOR126) with properties resembling the well-established multicopy vector pIJ702. Shuttle vectors capable of functioning in both and were also constructed. The region of pJVl essential for replication and maintenance was localized to a 2·5 kb segment. Stable maintenance of pWOR109 and pWOR12Q was observed in the presence of derivatives ofpIJl0l, the progenitor of pIJ702.

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1986-08-01
2021-08-04
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