The biovar host-range plasmid pJB5JI was transferred to six biovar strains. Inheritance of pJB5JI enabled the biovar strains to nodulate both peas and clover species. Bacteria were isolated from nodules formed on peas (the ‘correct’ host for biovar ) and the hosts for biovar (white, red and subterranean clover). Isolates from peas and white clover appeared to have lost or changed the host-range plasmid conferring the ability to nodulate white clover or peas respectively. Isolates from subterranean and red clover could often nodulate all host plants. These results show that the host exerts some form of functional incompatibility when interacting with hybrid strains and that some hosts are more stringent than others.


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