1887

Abstract

Summary: Immunoelectrophoresis, using antibodies raised against homogeneously pure nitrate reductase (NR; EC 1.6.6.2), was used to study the regulation of NR synthesis in . Extracts from ammonium-grown cultures did not contain cross-reacting material, but cross-reacting material was detected in cultures transferred to nitrogen-free and nitrate medium. The appearance of NR activity in these extracts appeared to be the result of protein synthesis. The possible role of nitrate in the stimulation of active NR synthesis is discussed. Losses of NR activity that occurred in nitrate-grown cultures transferred to ammonium medium correlated with decreases in cross-reacting material.

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1986-07-01
2021-08-04
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References

  1. Ali A. H. 1985 Nitrate assimilation in yeasts PhD thesis University of Wales; Swansea:
    [Google Scholar]
  2. Ali A. H., Hipkin C. R. 1985; Nitrate assimilation in the basidiomycete yeast Sporobolomyces roseus. Journal of General Microbiology 131:1867–1874
    [Google Scholar]
  3. Amy N. K., Garrett R. H. 1979; Immunoelectrophoretic determination of nitrate reductase in Neurospora crassa. Analytical Biochemistry 95:97–107
    [Google Scholar]
  4. Bahns M., Garrett R. H. 1980; Demonstration of de novo synthesis of Neurospora crassa nitrate reductase during induction. Journal of Biological Chemistry 255:690–693
    [Google Scholar]
  5. Bradford M. 1976; A rapid and sensitive method for the quantitation of micrograms of protein utilizing the principle of protein–dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  6. Funkhouser E. A., Ramadoss G. S. 1980; Synthesis of nitrate reductase (EC 1.6.6.1) in Chlorella vulgaris. II. Evidence for synthesis in ammonium grown cells. Plant Physiology 65:944–948
    [Google Scholar]
  7. Funkhouser E. A., Shen T. C., Ackerman A. R. 1980; Synthesis of nitrate reductase in Chlorella. I. Evidence for an inactive protein precursor. Plant Physiology 65:939–943
    [Google Scholar]
  8. Funkhouser E. A., Gewitz H. S., Vennesland B. 1983; Accumulation of demolybdo nitrate reductase during induction and its separation from active nitrate reductase in Chlorella. Plant and Cell Physiology 24:1565–1567
    [Google Scholar]
  9. Garrett R. H. 1972; Induction of nitrate reductase in Neurospora crassa. Biochimica et biophysica acta 264:481–489
    [Google Scholar]
  10. Hipkin C. R., Syrett P. J. 1977; Post-transcriptional control of nitrate reductase formation in green algae. Journal of Experimental Botany 28:1270–1277
    [Google Scholar]
  11. Hipkin C. R., Al-Bassam B. A., Syrett P. J. 1980; The role of nitrate and ammonium in the regulation of the development of nitrate reductase in Chlamydomonas reinhardii (2192). Planta 150:13–18
    [Google Scholar]
  12. Hipkin C. R., Hermann-Smith J. A., Syrett P. J. 1985; The role of nitrate, photosynthesis and protein turnover in the formation of nitrate reductase in the nit A mutant of Chlamydomonas. Biochimica et biophysica acta 838:191–196
    [Google Scholar]
  13. Hipkin C. R., Ali A. H., Cannons A. 1986; Structure and properties of assimilatory nitrate reductase from the yeast Candida nitratophila. Journal of General Microbiology 132:1997–2003
    [Google Scholar]
  14. Hynes M. J. 1973; The effect of lack of a carbon source on nitrate reductase activity in Aspergillus nidulans. Journal of General Microbiology 79:155–157
    [Google Scholar]
  15. Kinsky S. G. 1961; Induction and repression of nitrate reductase in Neurospora crassa. Journal of Bacteriology 82:898–904
    [Google Scholar]
  16. Lewis C. M., Fincham J. R. S. 1970; Regulation of nitrate reductase in the basidiomycete, Ustilago maydis. Journal of Bacteriology 103:55–61
    [Google Scholar]
  17. Ouchterlony O. 1953; Antigen–antibody reactions in gels. IV. Types of reactions in coordinated systems of diffusion. Acta pathologica et microbiologica scandinavica 32:231–240
    [Google Scholar]
  18. Scholl R. L., Harper J. E., Hageman R. H. 1974; Improvements of the nitrite color development in assays of nitrate reductase by phenazine methosulfate and zinc acetate. Plant Physiology 53:825–828
    [Google Scholar]
  19. Sorger G. J. 1965; Simultaneous induction and repression of nitrate reductase and TPN cytochrome c reductase in Neurospora crassa. Biochimica et biophysica acta 99:234–245
    [Google Scholar]
  20. Sorger G. J., Debanne M. T., Davies J. 1974; Effect of nitrate on the synthesis and decay of nitrate reductase of Neurospora. Biochemical Journal 140:395–403
    [Google Scholar]
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