Detection of a Species-specific Antigen of by Western Blot Analysis Free

Abstract

Summary: Western blot analysis was used to identify antigenic components of .Polypeptides bound to nitrocellulose membranes were probed with murine antisera raised to two strains of . and antibody-antigen complexes were detected with I-labelled antimouse immunoglobulin followed by autoradiography. Although there was inter-strain variation in immunogenic polypeptide profiles, all 23 strains of examined contained a common antigen of molecular mass 41 kDa. This antigen was not found in any of six other bacterial genera.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-7-1969
1986-07-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/7/mic-132-7-1969.html?itemId=/content/journal/micro/10.1099/00221287-132-7-1969&mimeType=html&fmt=ahah

References

  1. Dunkelberg W. E., Skaggs R., Kellogg D. S. 1970; Method for isolation and identification ofCorynebacterium vaginale (Haemophilus vaginalis).. Applied Microbiology 19:47–52
    [Google Scholar]
  2. Gardne H. L., Dukes C. D. 1955; Haemophilus vaginalis vaginitis A newly defined specific infection previously classified ‘non-specific’vaginitis. American Journal of Obstetrics and Gynecology 69:962–976
    [Google Scholar]
  3. Greenwood J. R., Pickett M. J. 1980; Transfer of Haemophilus vaginalis (Gardner and Dukes) to a new genus, Gardnerella: G. vaginalis (Gardner and Dukes) comb. nov. International Journal of Systematic Bacteriology 30:170–178
    [Google Scholar]
  4. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of 1311-labelled human growth hormone of high specific radioactivity. Biochemical Journal 89:114–123
    [Google Scholar]
  5. Laemmli U. K. 1970; Cleavage of structural protein during the asembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  6. Reyn A., Birch-Anderson A., Lapage S. P. 1966; An electron microscopic study of thin sections of Haemophilus vaginalis (Gardner and Dukes) and some possibly related species. Canadian Journal of Microbiology 12:1125–1136
    [Google Scholar]
  7. Smaron M. F., Vice J. L. 1974; Analysis of Corynebacterium vaginale by an immunodiffusion technique. Applied Microbiology 27:469–474
    [Google Scholar]
  8. Taylor E., Phillips I. 1983; The identification of Gardnerella vaginalis. Journal of Medical Microbiology 16:83–92
    [Google Scholar]
  9. Taylor-Robinson D. 1984; The bacteriology of Gardnerella vaginalis. In Bacterial Vaginosis, pp41–55 Edited By Màrdh P.-A., Taylor-Robinson D. Stockholm: Almquist & Wiksell;
    [Google Scholar]
  10. Towbin H., Strehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 79:4350–4354
    [Google Scholar]
  11. Wang S. P., Holmes K. K., Knapp J. S., Ott S., Kyzer D. 1977; Immunologic classification of Neisseria gonorrhoeae with micro-immunofluorescence. Journal of Immunology 119:795–803
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-7-1969
Loading
/content/journal/micro/10.1099/00221287-132-7-1969
Loading

Data & Media loading...

Most cited Most Cited RSS feed