1887

Abstract

Summary: A Transformant of carrying a recombinant plasmid, pLP11 (9.5 MDa), on which the penicillinase gene () and kanamycin resistance gene () were located was subjected to mutagenesis, and a mutant plasmid (9.5 MDa; ), designated pTRA 117, obtained. A transformant of carrying pTRA117 could grow at 63 °C in medium containing kanamycin, whereas a transformant carrying pLP11 could not. Although pTRA117 was deteeted as covalently closed circular (ccc) DNA when it was extracted from transformants cultured at 48 °C, it was integrated into the host chromosome when the culture temperature was shifted up to 63 °C. If the culture temperature was lowered to 48 °C from 63 °C, a new plasmid (10.7 MDa; ), designated pTRZ117, could be detected as ccc DNA, the size of this plasmid suggested that it was pTRA117 plus a 1.2 MDa DNA fragment of the host chromosome, and this was confirmed by Southern hybridization. pTRZ90 (7.9 MDa; ) was constructed from pTRZ117 by the deletion of a 2.8 MDa DNA fragment that contained . Fresh transformants of that carried either pTRZ117 or pTRZ90 could grow at 65 °C.

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/content/journal/micro/10.1099/00221287-132-7-1951
1986-07-01
2022-01-22
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References

  1. Aiba S., Koizumi J.-I. 1984; Effects of temperature on plasmid stability and penicillinase productivity in a transformant of Bacillus stearothermophilus.. Biotechnology and Bioengineering 26:1026–1031
    [Google Scholar]
  2. Fujii M., Imanaka T., Aiba S. 1982; Molecular cloning and expression of penicillinase genes from Bacillus licheniformis in the thermophile Bacillus stearothermophilus.. Journal of General Microbiology 128:2997–3000
    [Google Scholar]
  3. Imanaka T., Fujii M., Aiba S. 1981a; Isolation and characterization of antibiotic resistance plasmids from thermophilic bacilli and construction of deletion plasmids.. Journal of Bacteriology 146:1091–1097
    [Google Scholar]
  4. Imanaka T., Tanaka T., Tsunekawa H., Aiba S. 1981b; Cloning of the genes for penicillinase, penP and pen I, of Bacillus licheniformis in some vector plasmids and their expression in Escherichia coli, Bacillus subtilis, and Bacillus licheniformis . Journal of Bacteriology 147:776–786
    [Google Scholar]
  5. Imanaka T., Fujii M., Aramori I., Aiba S. 1982; Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis.. Journal of Bacteriology 149:824–830
    [Google Scholar]
  6. Imanaka T., Ano T., Fujii M., Aiba S. 1984; Two replication determinants of an antibiotic-resistance plasmid, pTB19, from a thermophilic bacillus.. Journal of General Microbiology 130:1399–1408
    [Google Scholar]
  7. Imanaka T., Himeno T., Aiba S. 1985; Effect of in vitro DNA rearrangement in the NH2-terminal region of the penicillinase gene from Bacillus licheniformis on the mode of expression in Bacillus subtilis.. Journal of General Microbiology 131:1753–1763
    [Google Scholar]
  8. Koizumi J.-I., Monden Y., Aiba S. 1985; Effects of temperature and dilution rate on the copy number of recombinant plasmid in continuous culture of Bacillus stearothermophilus (pLPll).. Biotechnology and Bioengineering 27:721–728
    [Google Scholar]
  9. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  10. Matsumura M., Katakura Y., Imanaka T., Aiba S. 1984; Enzymatic and nucleotide sequence studies of a kanamycin-inactivating enzyme encoded by a plasmid from thermophilic bacilli in comparison with that encoded by plasmid pUBl 10.. Journal of Bacteriology 160:413–420
    [Google Scholar]
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