1887

Abstract

Summary: To determine whether enzyme electrophoretic polymorphism in populations was influenced by environmental background, the mobilities of four electrophoretically variable esterases (A, B, C and I) were examined. The distinction between isolates was established by significant differences in the electrophoretic distribution and the genetic diversity coefficient of individual esterases. Principal components analysis on each population and on all strains revealed three groups of allozymes. The first, characterized by slow electrophoretic mobilities of esterase B, was frequently observed in strains obtained from human extra-intestinal infections and rarely in commensal organisms. The second, characterized by fast mobilities of esterases A and B, was frequently found in animal isolates. The third, characterized by prominence of the most common mobilities of esterases B and A, was recovered in all populations. These results were confirmed by discriminant analysis. Among the 610 strains investigated, 316 electrophoretic types (distinctive combinations of allozymes of the four varieties of esterases) were distinguished, illustrating high esterase polymorphism.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-7-1843
1986-07-01
2021-05-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/7/mic-132-7-1843.html?itemId=/content/journal/micro/10.1099/00221287-132-7-1843&mimeType=html&fmt=ahah

References

  1. Brenner D. J., Falkow S. 1971; Molecular relationships among members of the Enterobacteriaceae. Advances in Genetics 16:81–118
    [Google Scholar]
  2. Brenner D. J., Fanning G. R., Johnson K. E., Citarella R. V., Falkow S. 1969; Polynucleotide sequence relationships among members of Enterobacteriaceae. Journal of Bacteriology 98:637–650
    [Google Scholar]
  3. Brenner D. J., Fanning G. R., Skerman F. J., Falkow S. 1972; Polynucleotide sequence divergence among strains of Escherichia coli and closely related organisms. Journal of Bacteriology 109:637–650
    [Google Scholar]
  4. Dixon W. 1981 B.M.D.P. Statistical Software Berkeley, California: University of California Press;
    [Google Scholar]
  5. Gillespie J. H., Langley C. H. 1974; A general model to account for enzyme variation in natural populations. Genetics 76:637–650
    [Google Scholar]
  6. Goullet PH. 1973; An esterase zymogram of Escherichia coli . Journal of General Microbiology 77:637–650
    [Google Scholar]
  7. Goullet PH. 1980; Esterase electrophoretic pattern relatedness between Shigella species and Escherichia coli . Journal of General Microbiology 117:637–650
    [Google Scholar]
  8. Goullet PH. 1981; Characterization of Serratia odorifera, S. fonticola and S. ficaria by the electrophoretic patterns of their esterases. Journal of General Microbiology 127:637–650
    [Google Scholar]
  9. Goullet PH., Picard B. 1984a; Typage életro-phorétique des esterases d,Escherichia coli au cours de septicémies. La Presse Médicale 13:637–650
    [Google Scholar]
  10. Goullet PH., Picard B. 1984b; Distinctive electrophoretic and isoelectric focusing patterns of esterases from Yersinia enterocolitica and Yersinia pseudotuberculosis . Journal of General Microbiology 130:637–650
    [Google Scholar]
  11. Goullet PH., Picard B. 1985; A two-dimensional electrophoretic profile for bacterial esterases. Electrophoresis 6:637–650
    [Google Scholar]
  12. Goullet PH., Picard B. 1986; Highly pathogenic strains of Escherichia coli revealed by distinct electrophoretic pattern of carboxylesterase B. Journal of General Microbiology 132:637–650
    [Google Scholar]
  13. Goullet PH., Picard B., Toucas M. 1983; Différenciation du sérotype 6 des sérotypes 1-5 de Shigella flexneri par électrophorèse et focalisation isoélectrique de leurs estérases. Annales de microbiologie 134B:637–650
    [Google Scholar]
  14. Goullet PH., Picard B., Laget P. F. 1984; Purification and properties of carboxylesterase B of Escherichia coli . Annales de Microbiologie 135A:637–650
    [Google Scholar]
  15. Lennox E. S. 1955; Transduction of linked genetic characters of the host by bacteriophage PL. Virology 1:637–650
    [Google Scholar]
  16. Milkman R. 1973; Electrophoretic variation in Escherichia coli from natural sources. Science 182:637–650
    [Google Scholar]
  17. Nei M. 1975 Molecular Population Genetics and Evolution New York: Elsevier;
    [Google Scholar]
  18. Ochman H., Whittam T. S., Caugant D. A., Selander R. K. 1983; Enzyme polymorphism and genetic population structure in Escherichia coli and Shigella . Journal of General Microbiology 129:2715–2726
    [Google Scholar]
  19. Picard B., Goullet PH. 1985; Comparative electrophoretic profiles of esterases, and of glutamate, lactate and malate dehydrogenases, from Aeromonas hydrophila, A. caviae and A. sobria . Journal of General Microbiology 131:637–650
    [Google Scholar]
  20. Selander R. K., Levin B. R. 1980; Genetic diversity and structure in Escherichia coli populations. Science 210:637–650
    [Google Scholar]
  21. Whittam T. S., Ochman H., Selander R. K. 1983; Multilocus genetic structure in natural populations of Escherichia coli . Proceedings of the National Academy of Sciences of the United States of America 80:637–650
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-7-1843
Loading
/content/journal/micro/10.1099/00221287-132-7-1843
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error