@article{mbs:/content/journal/micro/10.1099/00221287-132-7-1805, author = "Cortés, Jesús and Liras, Paloma and Castro, José M. and Martín, Juan F.", title = "Glucose Regulation of Cephamycin Biosynthesis in Streptomyces lactamdurans is Exerted on the Formation of α-Aminoadipyl-cysteinyl-valine and Deacetoxycephalosporin C Synthase", journal= "Microbiology", year = "1986", volume = "132", number = "7", pages = "1805-1814", doi = "https://doi.org/10.1099/00221287-132-7-1805", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-132-7-1805", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Summary: Glucose exerted a concentration-dependent negative regulation on the biosynthesis of cephamycin C by Streptomyces lactamdurans. Formation of the cephamycin precursor δ(α-aminoadipyl)-cysteinyl-valine was greatly decreased by excess glucose. The ring-expanding enzyme deacetoxycephalosporin C synthase was strongly repressed by glucose in vivo. Isopenicillin N synthase (cyclase) and isopenicillin N epimerase were not repressed by glucose. However, the activity of isopenicillin N synthase was inhibited in vitro by glucose 6-phosphate, and the activity of deacetoxycephalosporin C synthase was inhibited by inorganic phosphate, glucose 6-phosphate, fructose 2,6-diphosphate and fructose 1,6-diphosphate. The intracellular cAMP content decreased as growth proceeded and remained lower in glucose-supplemented cells than in control cultures. cAMP did not seem to be involved in glucose control of cephamycin biosynthesis.", }