1887

Abstract

Summary: Initial attempts to grow N-fixing autotrophically on H, CO, O and N in a closed system yielded variable results. Poor growth was found to be due to rapid O depletion and the requirement for an agar surface. In a closed system, CH reduction assays could not be carried out due to complete consumption of H. Hence a flow-through culturing technique was developed to supply gases at a constant partial pressure and to perform CH reduction assays in a continuous flow system. Hydrogenase of autotrophic was not inhibited by CH, even at 0.5 atm, and the of hydrogenase for H was approximately 0.15 atm. The effects of O and H on CH reduction were examined, using the flow-through assay system. The rate of CH reduction decreased below 0.074 atm H, suggesting that ATP and reductant supply were limiting the nitrogenase activity.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-132-7-1797
1986-07-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/132/7/mic-132-7-1797.html?itemId=/content/journal/micro/10.1099/00221287-132-7-1797&mimeType=html&fmt=ahah

References

  1. Berndt H., Wölfle D. 1978; Hydrogenase: its role as electron generating enzyme in the nitrogen fixing - bacterium Xanthobacter autotrophicus. In Hydrogenases Their Catalytic Activity, Structure and Function pp. 327–351 Edited by Schlegel H. G., Schneider K. Göttingen: Erich Goltze;
    [Google Scholar]
  2. Bowien B., Schlegel H. G. 1981; Physiology and biochemistry of aerobic hydrogen-oxidizing bacteria. Annual Review of Microbiology 35:420–421
    [Google Scholar]
  3. Hanus F. J., Maier R. J., Evans H. J. 1979; Autotrophic growth of hydrogen-uptake positive strains of Rhizobium japonicum in an atmosphere supplied with hydrogen gas. Proceedings of the National Academy of Sciences of the United States of America 76:1788–1792
    [Google Scholar]
  4. Hill S., Postgate J. R. 1969; Failure of putative nitrogen fixing bacteria to fix nitrogen. Journal of General Microbiology 58:277–285
    [Google Scholar]
  5. Ooyama J. 1971; Simultaneous fixation of carbon dioxide and nitrogen in the presence of hydrogen and oxygen by a bacterium. Report of the Fermentation Research Institute3943–44
    [Google Scholar]
  6. Pedrosa F. O., DöbEreiner J., Yates M. G. 1980; Hydrogen-dependent growth and autotrophic carbon dioxide fixation in Derxia . Journal of General Microbiology 119:547–551
    [Google Scholar]
  7. Smith L. A., Hill S., Yates M. G. 1976; Inhibition by acetylene of conventional hydrogenase in nitrogen fixing bacteria. Nature, London 262:209–210
    [Google Scholar]
  8. Tchan Y. T., Jensen H. L. 1963; Studies of nitrogen fixing bacteria. VIII. Proceedings of the Linnean Society of New South Wales 38:379–385
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-132-7-1797
Loading
/content/journal/micro/10.1099/00221287-132-7-1797
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error