1887

Abstract

Summary: Growth of 12 strains in a broth medium with and without aeration was compared. In general, the aerated cultures grew faster and produced more biomass, at the expense of glucose and other sugars, than unaerated cultures. The more efficient growth correlated well with the production of acetate rather than ethanol as an end-product of metabolism in aerated cultures; unaerated cultures produced little or no acetate.

Mutants of X2 were isolated that had lost the capacity to be stimulated by aeration; they were completely deficient in NAD(P)H oxidase activity and did not accumulate acetate in aerobic cultures. Without NAD(P)H oxidase the mutants rely on the ethanol branch of the heterolactate pathway to regenerate NAD(P) from NAD(P)H, irrespective of the presence or absence of O. The presence of NAD(P)H oxidase in parental cultures allows them to utilize O as a terminal electron acceptor and produce more ATP per mol of sugar utilized when O is available than when it is limiting.

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1986-07-01
2021-10-27
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References

  1. Anders R. F., Hogg D. M., Jago G. R. 1970; Formation of hydrogen peroxide by group N streptococci and its effect on their growth and metabolism. Applied Microbiology 19:602–612
    [Google Scholar]
  2. Demoss R. D., Bard R. C., Gunsalus I. C. 1951; The mechanism of the heterolactic fermentation: a new route of ethanol formation. Journal of Bacteriology 62:499–511
    [Google Scholar]
  3. Dempsey P. M., O’Leary J., Condon S. 1975; Polarographic assay of hydrogen peroxide accumulation in microbial cultures. Applied Microbiology 29:170–174
    [Google Scholar]
  4. Fitzgerald F. M. 1983; Aerobic metabolism of group N streptococci and Leuconostoc mesenteroides. MSc thesis University College: Cork;
    [Google Scholar]
  5. Garvie E. I. 1969; Lactic dehydrogenases of strains of the genus Leticonostoc. Journal of General Microbiology 58:85–94
    [Google Scholar]
  6. Gawehn K., Bergmeyer H. U. 1974; D(−) Lactate. In Methods in Enzymatic Analysis pp. 1492–1495 Edited by Bergmeyer H. U. Weinheim: Verlag Chemie;
    [Google Scholar]
  7. Goldberg M., Fessenden J. M., Racker E. 1966; Phosphoketolase. Methods in Enzymology 9:515–520
    [Google Scholar]
  8. Ito S., Hashiba H., Eguchi Y. 1974; adaptive control of the ethanol forming system in heterolactic acid bacteria. Journal of Biochemistry 75:577–581
    [Google Scholar]
  9. Ito S., Kobayashi T., Ohta Y., Akiyama Y. 1983; Inhibition of glucose catabolism by aeration in Leuconostoc mesenteroides. Journal of Fermentation Technology 61:353–358
    [Google Scholar]
  10. Johnson M. K., Mccleskey C. S. 1957; Studies on the aerobic carbohydrate metabolism of Leuconostoc mesenteroides. Journal of Bacteriology 74:22–25
    [Google Scholar]
  11. Johnson M. K., Mccleskey C. S. 1958; Further studies on the aerobic metabolism of leuconostoc mesenteroides. Journal of Bacteriology 75:98–101
    [Google Scholar]
  12. Kawai K., Yashima S., Okami Y., Sasaki Y. 1971; Aerobic dissimilation of glucose by heterolactic bacteria. 1. Reduced pyridine nucleotide-oxidising enzymes in Leuconostoc mesenteroides. Journal of General and Applied Microbiology 17:51–62
    [Google Scholar]
  13. Keenan T. W. 1968; Production of acetic acid and other volatile compounds by Leuconostoc citrovorum and Leuconostoc dextranicum. Applied Microbiology 16:1881–1885
    [Google Scholar]
  14. Lees G. J., Jago G. R. 1976; Acetaldehyde: an intermediate in the formation of ethanol from glucose by lactic acid bacteria. Journal of Dairy Research 43:63–73
    [Google Scholar]
  15. Lucey C. A. 1985; Active role of oxygen and NADH oxidase in growth and energy metabolism of Leuconostoc. MSc thesis University College: Cork;
    [Google Scholar]
  16. De MAN J. C., Rogosa M., Sharpe M. E. 1960; A medium for the cultivation of lactobacilli. Journal of Applied Bacteriology 23:130–135
    [Google Scholar]
  17. Murphy M. G., Condon S. 1984; Comparison of aerobic and anaerobic growth of Lactobacillus plantarum in a glucose medium. Archives of Microbiology 138:49–53
    [Google Scholar]
  18. Murphy M. G., O’ CONNOR L., Walsh D., Condon S. 1985; Oxygen dependent lactate utilization by Lactobacillus plantarum. Archives of Microbiology 141:75–79
    [Google Scholar]
  19. Stadtman E. R., Burton R. M. 1957; Aldehyde dehydrogenase from Clostridium kluyveri. Methods in Enzymology 1:518–523
    [Google Scholar]
  20. Thornhill P. J., Cogan T. M. 1984; The use of gas-liquid chromatography to determine the end-products of growth of lactic acid bacteria. Applied and Environmental Microbiology 47:1250–1254
    [Google Scholar]
  21. Whittenbury R. 1963; The use of soft agar in the study of conditions affecting the utilization of fermentation substrates by lactic acid bacteria. Journal of General Microbiology 32:375–384
    [Google Scholar]
  22. Whittenbury R. 1966; A study of the genus Leuconostoc. Archives of Microbiology 53:317–327
    [Google Scholar]
  23. Yashima S., Kawai K., Okami Y., Sasaki Y. 1970; Effect of oxygen on glucose dissimilation by heterolactic bacteria. Journal of General and Applied Microbiology 16:543–545
    [Google Scholar]
  24. Yashima S., Kawai K., Kazahaya T., Okami Y., Sasaki Y. 1971; Aerobic dissimilation of glucose by heterolactic bacteria. II. Phosphate acetyltrans-ferase of Leuconostoc mesenteroides. Journal of General and Applied Microbiology 17:173–183
    [Google Scholar]
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