1887

Abstract

Specific monoclonal antibody and Western blot analysis were used to examine the phenotypic expression of the major 47 kDa surface immunogen of among organisms cultivated . Tissue-cultured treponemes synthesized the 47 kDa immunogen as well as, or better than, organisms cultivated in vivo (rabbit testicles).

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/content/journal/micro/10.1099/00221287-132-6-1775
1986-06-01
2024-11-13
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References

  1. ALDERETE J.F., BASEMAN J.B. 1979; Surfaceassociated host proteins on virulent Treponema pallidum. Injection and Immunity 26:1048–1056
    [Google Scholar]
  2. BISHOP N.H., MILLER J.N. 1976; Humoral immunity in experimental syphilis. 11. The relationship of neutralizing factors in immune serum to acquired resistance. Journal of’lmmunology 117:197–207
    [Google Scholar]
  3. CHRISTIAN S. 1963; Protective layer covering pathogenic treponemata. Lancet 1:423–425
    [Google Scholar]
  4. FIELDSTEL A.H., Cox D.L., MOECKLI R.A. 1981; Cultivation of virulent Treponemapallidum in tissue culture. Injection and Immunity 32:908–915
    [Google Scholar]
  5. FIELDSTEEL A.H., Cox, D.L., MOECKLI R.A. 1982; Further studies on replication of virulent Treponema pallidum in tissue cultures of Sfl Ep cells.. Injection and Immunity 35:449–445
    [Google Scholar]
  6. JONES S.A., MARCHITTO K.S., MILLER J.N., NORGARD M.V. 1984; Monoclonal antibody with hemagglutination, immobilization, and neutralization activities defines an immunodominant, 47,000 mol wt, surface-exposed immunogen of Treponema pallidum (Nichols).. Journal of Experimental Medicine 160:1404–1420
    [Google Scholar]
  7. LOGAN L.C. 1974; Rabbit globulin and antiglobulin factors associated with Treponemapallidum grown in rabbits. British Journal of’ Venereal Disease 50:421–427
    [Google Scholar]
  8. MARCHITO K.S., JONES S.A., SCHELL R.F., HOLMANS P.L., NORGARD M.V. 1984; Monoclonal antibody analysis of specific antigenic similarities among pathogenic Treponema pallidum subspecies.. Injection and Immunity 45:660–666
    [Google Scholar]
  9. MARCHITTO K.S., SELLAND-GROSSLING C.K., NORGARD M.V. 1986; Molecular specificities of monoclonal antibodies directed against virulent Treponema pallidum.. Infection and Immunity 51:168–178
    [Google Scholar]
  10. MOSKOPHIDIS M., MULLER F. 1984; Molecular analysis of immunoglobulins M and G immune response to protein antigens of Treponema pallidum in human syphilis.. Infection and Immunity 43:127–132
    [Google Scholar]
  11. NORRIS S.J. 1982; In vitro cultivation of Treponema pallidum : independent confirmation. Infection and Immunity 36:437–439
    [Google Scholar]
  12. PENN C.W., COCKAYE A., BAILEY M.J. 1985; The outer membrane of Treponemapallidum : biological significance and biochemical properties.. Journal of General Microbiology 131:2349–2357
    [Google Scholar]
  13. ROBERTSON S.M., KETTMAN J.R., MILLER J.N., NORGARD M.V. 1982; Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols).. Injection and Immunity 36:1076–1083
    [Google Scholar]
  14. STAMM L.V., BASSFORD P.J.JR. 1985; Cellular and extracellular protein antigens of Treponema pallidum synthesized during in vitro incubation of freshly extracted organisms.. Infection and Immunity 47:799–807
    [Google Scholar]
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